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Allergologia et Immunopathologia
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Inicio Allergologia et Immunopathologia Sinomenine ameliorates the airway remodelling, apoptosis of airway epithelial ce...
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Vol. 46. Num. 1.January - February 2018
Pages 1-104
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Vol. 46. Num. 1.January - February 2018
Pages 1-104
Original Article
DOI: 10.1016/j.aller.2017.05.004
Sinomenine ameliorates the airway remodelling, apoptosis of airway epithelial cells, and Th2 immune response in a murine model of chronic asthma
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S. Işıka,
Corresponding author
drsakinekar83@hotmail.com

Corresponding author.
, M. Karamanb, S.Ç. Micilic, Ş. Çağlayan-Sözmena, H.A. Bağrıyanıkc, Z. Arıkan-Ayyıldıza, N. Uzunera, Ö. Karamana
a Dokuz Eylul University, Department of Pediatric Allergy and Immunology, Izmir, Turkey
b Dokuz Eylul University, Department of Microbiology, Izmir, Turkey
c Dokuz Eylul University, Department of Histology, Izmir, Turkey
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Tables (4)
Table 1. Comparison of the histopathological findings of the study groups (mean±SD).
Table 2. Comparison of the immunohistochemical scoring of the study groups (mean±SD).
Table 3. Comparison of cytokine levels in lung homogenates and OVA-Specific IgE in Serum (mean±SD).
Table 4. Comparison of eosinophil counts in BAL fluid (mean±SD).
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Abstract
Background

Sinomenine (SIN), an alkaloid isolated from the root of Sinomenium acutum which has a variety of pharmacological effects, including anti-inflammation, immunosuppression and anti-angiogenesis. The present study aimed to evaluate the effects of SIN on airway remodelling, epithelial apoptosis, and T Helper (Th)-2 derived cytokine levels in a murine model of chronic asthma.

Methods

Twenty-two BALB/c mice were divided into four groups; I (control), II (placebo), III, IV. Mice in groups III and IV received the SIN (100mg/kg), and dexamethasone (1mg/kg) respectively. Epithelium thickness, sub-epithelial smooth muscle thickness, number of mast and goblet cells of samples isolated from the lung were measured. Immunohistochemical scorings of the lung tissue for matrix metalloproteinase-9 (MMP-9), vascular endothelial growth factor (VEG-F), transforming growth factor-beta (TGF-β), terminal deoxynucleotidyl transferase-mediated dUTP nick endlabeling (TUNEL) and cysteine-dependent aspartate-specific proteases (caspase)-3 were determined. IL-4, IL-5, IL-13, Nitric oxide in bronchoalveolar lavage fluid (BALF) and ovalbumin-specific immunoglobulin (Ig) E in serum were quantified by standard ELISA protocols.

Results

The dose of 100mg/kg SIN treatment provided beneficial effects on all of the histopathological findings of airway remodelling compared to placebo (p<0.05). All cytokine levels in BALF and serum and immunohistochemical scores were significantly lower in 100mg/kg SIN treated group compared to the placebo (p<0.05).

Conclusions

These findings suggested that the dose of 100mg/kg SIN improved all histopathological changes of airway remodelling and its beneficial effects might be related to modulating Th-2 derived cytokines and the inhibition of apoptosis of airway epithelial cells.

Keywords:
Asthma
Airway remodelling
Angiogenesis
Apoptosis
Murine model
Sinomenine
Th2

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