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Información de la revista
Vol. 46. Núm. 3.
Páginas 269-270 (Julio - Septiembre 2014)
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Vol. 46. Núm. 3.
Páginas 269-270 (Julio - Septiembre 2014)
Imagen microbiológica
DOI: 10.1016/S0325-7541(14)70081-1
Open Access
Campylobacter fetus subsp. venerealis adhesion to MDBK cells
Adhesión de Campylobacter fetus subsp. venerealis a células MDBK
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María L. Chiapparronea,b,
Autor para correspondencia
mlchiapp@vet.unicen.edu.ar

Autor para correspondencia.
, Pedro E. Moránc, Hilda M. Echevarríab, Pedro Sotob, Fernando A. Paolicchid, María Catenab
a Consejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, República Argentina
b Laboratorio de Microbiología Clínica y Experimental, NACT, SAMP, Unidad Ejecutora de CONICET (CIVETAN), Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Tandil, Provincia de Buenos Aires, República Argentina
c Laboratorio de Virología, SAMP, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Tandil, Provincia de Buenos Aires
d Instituto Nacional de Tecnología Agropecuaria, EEA Balcarce, Departamento de Producción Animal, Laboratorio de Bacteriología, Balcarce, Provincia de Buenos Aires
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Members of the genus Campylobacter are recognized as causative agents of infectious diseases in humans and animals throughout the world. These bacteria are curved to spiral rods and have a single polar flagellum. Campylobacter fetus subsp. venerealis and Campylobacter fetus subsp. fetus are the causative agents of bovine genital campylobacteriosis, one of the most important venereal diseases in Argentina, which characteristically produces embryonic death and occasional abortion1.

Regarding Campylobacter pathogenesis, there is limited knowledge about the mechanisms involved in host-bacteria interaction in the venereal environment, adhesion, chemotaxis or tissue tropism4.

Campylobacter fetus is highly adapted to mucosal surfaces. Bacterial adhesion is an important initial step in infection. Pathogens use surface-located adhesins to interact with specific host cell receptors. Although some bacterial structures involved in the adhesion process are still unknown, there is evidence that the lipopolysaccharide and the flagellum participate in bacterial adherence. The flagellum also enables bacterial movement through the mucus4.

The challenge of cell lines such as HeLa, Hep-2, CHO, VERO, MDBK has allowed the evaluation of different species of Campylobacter strains isolated from humans and animals3,5. Cell adhesion and invasion for different Campylobacter species have been confirmed; however invasion has not been demostrated for C. fetus2.

C. fetus subsp. venerealis adhesion to MDBK cells was analyzed and confirmed by optical microscopy (Figs. 1 and 2) and scanning electron microscopy (Figs. 3 and 4). It was observed that the bacterium attaches by the apical portion involving the flagellum in the adhesion, which could indicate the presence of adhesins on it.

Figures 1 and 2.

Observation of Campylobacter fetus subsp. venerealis adhered to MDBK cells. Giemsa stain, 100X.

(0,26MB).
Figures 3 and 4.

Observation by scanning electron microscopy of Campylobacter fetus subsp. venerealis adhered to MDBK cells.

(0,14MB).
References
[1.]
D.J. Brenner, N.R. Krieg, J.T. Staley.
Campylobacter.
Bergey's Manual of Systematic Bacteriology,, 2, pp. 1145-1160
[2.]
Catena M. Campilobacteriosis genital bovina: inmunopatogenia de la mortalidad embrionaria. Tesis doctoral para obtener el grado académico de Doctor en Ciencia Animal. 2002. Facultad de Ciencias Veterinarias. UNCPBA.
[3.]
Chiapparrone ML, Catena M, Rodríguez E, Echevarría H, Monteavaro C, Morán P, Arroyo G, Soto P. Evaluación de la adhesión de Campylobacter fetus venerealis en diferentes líneas celulares. XIII Jornadas Argentinas de Microbiología, 2008, p. 181, Rosario. Santa Fe, Argentina.
[4.]
L.A. Joens, F. Haesebrouck, F. Pasmans.
Campylobacter and Helicobacter.
Pathogenesis of bacterial infections in animals., 4, pp. 483-501
[5.]
K.N. Prasad, T.N. Dhole, A. Ayyagari.
Adherence, invasion and cytotoxin assay of Campylobacter jejuni in HeLa and HEp-2 Cells.
J Diarrhoeal Dis Res., 4 (1996), pp. 255-259
Copyright © 2014. Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.
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