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Inicio Allergologia et Immunopathologia The signal transducer and activator of transcription 6 (STAT-6) mediates Th2 inf...
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Vol. 47. Issue 6.
Pages 535-543 (November - December 2019)
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Vol. 47. Issue 6.
Pages 535-543 (November - December 2019)
Original Article
DOI: 10.1016/j.aller.2019.02.006
The signal transducer and activator of transcription 6 (STAT-6) mediates Th2 inflammation and tissue damage in a murine model of peanut-induced food allergy
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C.R. Cardosoa,
Corresponding author
cristina@fcfrp.usp.br

Corresponding author.
, P.R. Provinciattob, D.F. Godoic, M.T. Fonsecab, B.R. Ferreirad, G. Teixeirae, F.Q. Cunhaf, C.F. Pinzanb, J.S. da Silvab
a Department of Clinical Analysis, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil
b Department of Biochemistry and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil
c Department of Internal Medicine, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil
d Department of Maternal and Child and Public Health Nursing, Ribeirão Preto School of Nursing, University of São Paulo, Ribeirão Preto, SP, Brazil
e Biology Institute, Fluminense Federal University, Niterói, RJ, Brazil
f Department of Pharmacology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil
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Table 1. Primers sequences used in real-time PCR assays.
Abstract
Introduction

Food allergies are inflammatory conditions mediated by Th2 and probably STAT-6 dependent immune responses.

Objective and design

Here we investigated the role of Signal Transducer and Activator of Transcription 6 (STAT-6) in development of inflammation in peanut allergy.

Methods

To induce food allergy, wild-type (WT) and mice deficient for STAT-6 (Stat6−/−) were sensitized with peanut proteins and challenged with peanut seeds.

Results

WT animals lost weight and refused the peanut diet, in contrast to Stat6−/− mice, which had a better maintenance of body weight and more regular seeds’ consumption. The augmented peanut-specific IgG, IgG1 and IgE in the allergic WT was abolished in Stat6−/− animals that also presented increased IgG2a. There was an overall reduction in the gut mediators in the absence of STAT-6, including those related to inflammatory and Th2 responses, in contrast to a rising counter regulatory and Th1 reaction in Stat-6−/− mice. These animals had IFN-γ and IL-10 similar to WT after the four-week challenge. Most interestingly, Stat-6−/− mice had no intestinal damage, in contrast to WT animals, which had inflammatory infiltrate, tissue destruction, epithelial exulceration, edema, congestion and loss of villous architecture in the small gut segments.

Conclusions

STAT-6 plays an important role in the establishment of the Th2 inflammatory responses and intestinal damage in peanut allergy.

Keywords:
Gut inflammation
Food allergy
STAT-6

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