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Inicio Allergologia et Immunopathologia Anti-allergic function of α-Tocopherol is mediated by suppression of PI3K-PKB a...
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Vol. 48. Issue 4.
Pages 395-400 (July - August 2020)
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Vol. 48. Issue 4.
Pages 395-400 (July - August 2020)
Original Article
DOI: 10.1016/j.aller.2019.11.005
Anti-allergic function of α-Tocopherol is mediated by suppression of PI3K-PKB activity in mast cells in mouse model of allergic rhinitis
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Geping Wua,b,
Corresponding author
Gordon_wu@qq.com

Corresponding author at: Department of Otolaryngology, Affiliated ZhangJiaGang Hospital of Soochow University, Suzhou 215600, China.
, Hongyan Zhub, Xinyang Wuc, Lili Liub, Xingkai Maa, Yifang Yuana, Xingli Fud, Ling Zhangb, Yan Lvb, Di Lib, Jianyong Liua, Jianbin Lua, Yan Yua, Menglin Lia
a Department of Otolaryngology, Affiliated ZhangJiaGang Hospital of Soochow University, Suzhou, 215600, China
b Institute of Translational Medicine, Affiliated ZhangJiaGang Hospital of Soochow University, Suzhou, 215600, China
c School of Ophthalmology and Optometry and Eye Hospital, Wenzhou Medical University, 325003, China
d Jiangsu University Health Science Center, Zhenjiang, Jiangsu Province, 212001, China
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Abstract
Background

Alpha-Tocopherol (α-TCP), one major form of vitamin E, has been known as a treatment for airway allergic inflammation. However, the role and mechanism of α-TCP in treating allergic rhinitis remains unclear.

Objective

In this study we examined the inhibitory function of α-TCP in a mouse model of allergic rhinitis.

Methods

Allergic phenotype was examined by hematoxylin and eosin staining. Total IgE, OVA-specific IgE, OVA-specific IgG1 and OVA-specific IgG2a levels were examined by ELISA. mRNA expression was measured by qPCR, protein levels were examined by Western Blot.

Results

Histological analysis of the nasal membranes revealed that there was a significant reduction in inflammatory cells appearance in cross-sections in alpha-TCP treatment of Ovalbumin (OVA)-sensitized mice compared to OVA sensitized animals. In addition, eosinophils were significantly reduced in nasal mucosa of alpha-TCP treatment of OVA-sensitized mice compared to the OVA group. Lower total IgE, OVA-specific IgE, OVA-specific IgG1 and OVA-specific IgG2a levels were found in alpha-TCP treatment of OVA-sensitized mice compared to the OVA group. Furthermore, we found that the subepithelial distribution of tryptase positive mast cells was reduced in the alpha-TCP treatment of OVA-sensitized mice. More importantly, the PI3K-PKB pathway was suppressed by α-TCP in mast cells.

Conclusions

Our results demonstrated that α-TCP-mediated suppression of PI3K-PKB activity in mast cells is a potential mechanism of anti-allergic function of α-TCP.

Keywords:
α-TCP
Allergic rhinitis
PI3K-PKB
Animal model
OVA

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