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Inicio Allergologia et Immunopathologia Ameliorating effects of Nigella sativa oil on aggravation of inflammation, oxida...
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Vol. 46. Issue 5.
Pages 472-481 (September - October 2018)
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Vol. 46. Issue 5.
Pages 472-481 (September - October 2018)
Original Article
DOI: 10.1016/j.aller.2018.02.005
Ameliorating effects of Nigella sativa oil on aggravation of inflammation, oxidative stress and cytotoxicity induced by smokeless tobacco extract in an allergic asthma model in Wistar rats
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T. Khaldi, N. Chekchaki, M. Boumendjel, F. Taibi, M. Abdellaoui, M. Messarah, A. Boumendjel
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Laboratory of Biochemistry and Environmental Toxicology, Department of Biochemistry, Faculty of Sciences, Badji Mokhtar University, Annaba, Algeria
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Tables (3)
Table 1. Cell numbers and total proteins levels in BALF of sensitized and treated rats.
Table 2. Antioxidant defense status in lung and erythrocytes of treated and control rats.
Table 3. Semi-quantitative scoring of architectural damage on histopathological examination of the rat lungs in the different treatment groups.
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Abstract
Background

The comparison of smokeless tobacco (ST) exposure versus Ovalbumin (Ova) sensitized rats or asthmatic patients has hardly been studied in the literature. Thus, the present study aims to investigate the aggravation of inflammation, exacerbation of asthma, oxidative stress and cytotoxicity induced by ST.

Methods

ST was given at the dose of 40mg/kg in an allergic asthma model in Wistar rats. Furthermore, the effects of oral administration of Nigella sativa oil (NSO), at a dose of 4mL/kg/day, were investigated.

Results

The obtained results showed that ST clearly enhanced lung inflammation through interleukin-4 (IL-4) and Nitric oxide (NO) increased production. Actually, ST was found to intensify the oxidative stress state induced by Ova-challenge in rats, which was proven not only by augmenting lipid peroxidation and protein oxidation, but also by altering the non-enzymatic and enzymatic antioxidant status. Furthermore, the aggravation of inflammation and oxidative stress was obviously demonstrated by the histopathological changes observed in lung. In contrast, NSO administration has shown anti-inflammatory effects by reducing IL-4 and NO production, restoring the antioxidant status, reducing lipid peroxidation and improving the histopathological alterations by both protein oxidation and NSO treatment.

Conclusions

Our data have proven that severe concurrent exposure to allergen and ST increases airway inflammation and oxidative stress in previously sensitized rats. They also suggest that the oral NSO treatment could be a promising treatment for asthma.

Keywords:
Smokeless tobacco
Experimental asthma
Nigella sativa
Airway inflammation
Oxidative stress
Wistar rats
Abbreviations:
AHR
b.w.
BALF
CAT
DGRSDT
DNPH
DTNB
ELISA
GPx
GSH
IFN-γ
IgE
IL-4
LD50
LPO
MDA
NBT
NO
NPSH
NSO
ONAB
Ova
PBS
ROS
SEM
SOD
ST
STE
TBA
TBARS
Th1
Th2
TQ

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