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Inicio Allergologia et Immunopathologia Evaluation of serum IgE in peach-allergic patients with systemic reaction by usi...
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Vol. 46. Issue 5.
Pages 482-490 (September - October 2018)
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Vol. 46. Issue 5.
Pages 482-490 (September - October 2018)
Original Article
DOI: 10.1016/j.aller.2018.02.007
Evaluation of serum IgE in peach-allergic patients with systemic reaction by using recombinant Pru p 7 (gibberellin-regulated protein)
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Y. Moria,1,
Corresponding author
yuji0319@fujita-hu.ac.jp

Corresponding author.
, F. Okazakib,1, C. Inuoa, Y. Yamaguchic, S. Masudad, S. Sugiurae, T. Fukuief, M. Nagaog, I. Tsugea, T. Yosikawaa, A. Yagamih, K. Matsunagai, T. Fujisawag, K. Itoe, H. Naritaj, Y. Kondok, Fruits Allergy Component Study Group, Japan
a Department of Pediatrics, School of Medicine, Fujita Health University, Aichi, Japan
b Department of Food Science and Human Nutrition Faculty of Agriculture, Ryukoku University, Kyoto, Japan
c Kyoto College of Nutritional and Medical Science, Kyoto, Japan
d Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, Japan
e Department of Allergy, Aichi Children's Health and Medical Center, Aichi, Japan
f Department of Pediatrics, Hamamatsu University School of Medicine, Shizuoka, Japan
g Allergy Center, Mie National Hospital, Mie, Japan
h Department of Allergology, Fujita Health University Second Educational Hospital, Aichi, Japan
i Department of Integrative Medical Science for Allergic Disease, Fujita Health University School of Medicine, Aichi, Japan
j Department of Food Science, Kyoto Women's University, Kyoto, Japan
k Department of Pediatrics, Fujita Health University Second Educational Hospital, Aichi, Japan
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Table 1. Background of subjects.
Abstract
Background

Lipid transfer protein (LTP) is a major fruit allergen. It has, however, recently been revealed that the systemic reaction in peach-allergic patients is related not only to LTP (Pru p 3) but also to gibberellin-regulated protein (Pru p 7). We investigated recombinant Pru p 7 (rPru p 7) for its potential use in worldwide standardization for the diagnosis of peach allergy.

Methods

Natural Pru p 7 (nPru p 7) was purified from peach crude extract using a monoclonal antibody affinity column. Complementary DNA for Pru p 7 was cloned and expressed in Escherichia coli and Pichia pastoris. Serum immunoglobulin (Ig) E in peach-allergic patients was examined by enzyme-linked immunosorbent assay (ELISA) using nPru p 7 and rPru p 7 (E. coli product: erPru p 7 and P. pastoris product: prPru p 7).

Results

Peach-allergic patients (n=27) were diagnosed and categorized into oral reaction (n=10) or systemic reaction (n=17). The nPru p 7 positivity based on serum IgE levels was 52% in the systemic-reaction group and 0% in the oral-reaction group (P<0.05). In the systemic-reaction group, there was no significant difference in reactivity between nPru p 7 and prPru p 7, but the reactivity of erPru p 7 was significantly lower than those of nPru p 7 and prPru p 7 (P<0.05).

Conclusions

We found that prPru p 7 exhibited reactivity in ELISA comparable to that of nPru p 7 for the diagnosis of peach allergy with systemic reaction.

Keywords:
Component-resolved diagnosis (CRD)
Fruit allergy
Gibberellin-regulated protein
Lipid transfer protein (LTP)
Peach
Pichia pastoris
Recombinant allergen
Abbreviations:
cDNA
CRD
DTT
ELISA
FDEIA
GRP
Ig
LTP
PBS
PCR
PFAS
OR
SR
SDS-PAGE

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