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Vol. 16. Issue 2.
Pages 50-58 (April - June 2018)
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Vol. 16. Issue 2.
Pages 50-58 (April - June 2018)
Original
Association of open field behavior with blood and semen characteristics in roosters: an alternative animal model
Asociación entre la conducta de gallos en campo abierto con sus características de sangre y de semen: un modelo animal alternativo
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Mohammad Mehdi Ommatia,b, Reza Heidaric, Mohammad Javad Zamiria,
Corresponding author
mjzamiri@gmail.com

Corresponding author.
, Sajjad Shojaeea, Amir Akhlaghia, Samira Sabourid
a Department of Animal Sciences, College of Agriculture, Shiraz University, Shiraz, Iran
b Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, People's Republic of China
c Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
d Department of Clinical Psychology, Roudehen branch, Islamic Azad University, Roudehen, Iran
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Tables (7)
Table 1. Correlation coefficients of open field behavioral parameters with blood and sperm parameters.
Table 2. Relationship of blood and sperm parameters with pace-bout, peck-bout and pace number in roosters (Mean±SE; n=108).
Table 3. Relationship of blood and sperm parameters with first pace, peck-number, and first-peck in roosters (Mean±SE; n=108).
Table 4. Relationship of blood and sperm parameters with crow-number and coming-forward in roosters (Mean±SE; n=108).
Table A.1. Ingredients and chemical composition of the basal diet (DM basis).
Table A.2. Behavioral ethogram.
Table A.3. The multivariate regression of open field test with blood and seminal characteristics.
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Abstract
Introduction

The paucity of literature is addressed regarding the correlation between open field as an individual behavioral trait on reproductive capacity in animals.

Materials and methods

To address this, Nine-month-old indigenous roosters were housed in individual cages. Each animal was observed twice a week for ten minutes before feeding in an open field apparatus for two weeks (7:00–12:00PM).

Results

Interestingly, it was found that rooster's semen characteristics were correlated with their open field behavior. On the other hand, plasma glucose level as a blood attribute was more correlated with semen characteristics. The open field monitoring also revealed that the roosters with the lowest delay to their first pace had the highest sperm forward motility and lower sperm abnormality. The heterophil to lymphocyte ratio (H:L) was found to be low when pace bout and pace numbers were 20 and 35, respectively. The negative correlation between H:L ratio and semen characteristics (live sperm percentage, sperm concentration, and membrane integrity) may be an indication of poor reproductive performance in fearful roosters with higher H:L ratio.

Conclusions

The data suggested a relationship between open field behavior indices and some reproductive parameters in roosters. The results might be applicable for selection of more reproductive animals. Hence, the rooster may also be useful model for similar studies in other species.

Keywords:
Blood attributes
Fear response
H:L ratio
Open field
Semen characteristics
Resumen
Introducción

Existe poca literatura que analice la relación entre el comportamiento individual en animales en campo abierto y su capacidad reproductiva.

Material y métodos

Para cubrir esta laguna se trabajó con gallos de 9 meses de edad que fueron encerrados en jaulas individuales. Cada animal se observó 2 veces a la semana, durante 10min, en una zona de campo abierto durante 2 semanas, antes de la alimentación (07:00-12:00 p.m.).

Resultados

Curiosamente, se encontró que la característica del semen de los gallos se correlacionaba con su comportamiento en campo abierto. Por otro lado, el nivel de glucosa en plasma, como un atributo de la sangre, se correlacionaba con las características del semen. La monitorización en campo abierto también reveló que los gallos con la menor demora en su primer canto tuvieron la mayor motilidad de los espermatozoides y la menor alteración del esperma. El nivel más bajo de la relación entre heterófilos y linfocitos (H: L) fue encontrado cuando los números de cantos y el ritmo eran 20 y 35, respectivamente. La correlación negativa entre el índice H:L y las características del semen (porcentaje de espermatozoides vivos, concentración de espermatozoides, e integridad de la membrana) puede ser un indicio de mal desempeño reproductivo de gallos con mayor índice H:L.

Conclusiones

Los datos indican que existe relación entre los índices de comportamiento en campo abierto y algunos parámetros reproductivos de los gallos. Los resultados podrían ser aplicables para la selección de los animales más reproductivos. El gallo también puede ser útil como un modelo para estudios similares en otras especies.

Palabras clave:
Atributos de sangre
Respuestas de miedo
Relación entre heterófilos y linfocitos
Campo abierto
Características del semen

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