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En localización interna a la retina se visualizan otras imágenes lineales móviles igualmente fijas al disco óptico sugestivas de membranas residuales de la endoftalmitis.</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Domingo Fernández-Vecilla, Iris Sharon Pérez-Ramos, Cristina Aspichueta-Vivanco, Miren Josebe Unzaga-Barañano" "autores" => array:4 [ 0 => array:2 [ "nombre" => "Domingo" "apellidos" => "Fernández-Vecilla" ] 1 => array:2 [ "nombre" => "Iris Sharon" "apellidos" => "Pérez-Ramos" ] 2 => array:2 [ "nombre" => "Cristina" "apellidos" => "Aspichueta-Vivanco" ] 3 => array:2 [ "nombre" => "Miren Josebe" "apellidos" => "Unzaga-Barañano" ] ] ] ] ] "idiomaDefecto" => "es" "Traduccion" => array:1 [ "en" => array:9 [ "pii" => "S2529993X22001411" "doi" => "10.1016/j.eimce.2022.06.011" "estado" => "S300" "subdocumento" => "" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:1 [ "total" => 0 ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2529993X22001411?idApp=UINPBA00004N" ] ] "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0213005X22000179?idApp=UINPBA00004N" "url" => "/0213005X/0000004000000010/v1_202212020757/S0213005X22000179/v1_202212020757/es/main.assets" ] ] "itemAnterior" => array:18 [ "pii" => "S2529993X2200243X" "issn" => "2529993X" "doi" => "10.1016/j.eimce.2021.12.016" "estado" => "S300" "fechaPublicacion" => "2022-12-01" "aid" => "2542" "copyright" => "Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica" "documento" => "simple-article" "crossmark" => 1 "subdocumento" => "crp" "cita" => "Enferm Infecc Microbiol Clin. 2022;40:586-7" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:1 [ "total" => 0 ] "en" => array:11 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Scientific letter</span>" "titulo" => "Possible intrauterine transmission of SARS-CoV-2: Ultrasound findings and viral molecular detection in amniotic fluid" "tienePdf" => "en" "tieneTextoCompleto" => "en" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "586" "paginaFinal" => "587" ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Posible transmisión intrauterina del SARS-CoV-2: Hallazgos ecográficos y detección molecular viral en líquido amniótico" ] ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0005" "etiqueta" => "Fig. 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 774 "Ancho" => 2007 "Tamanyo" => 246414 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Ultrasound images at 25 weeks of gestation showing liver (A) and placental (B) calcifications. At 28 weeks of gestation, there was progression of calcifications and changes in placental shape (C). At 36 weeks of pregnancy, the patient presented amniochorial detachment (D). Two days after birth, the child had persistent calcifications in the right and left liver lobes (E).</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Maria Carolina Andrade Maia, Carolina Santos Souza Tavares, Cliomar Alves dos Santos, Paulo Ricardo Martins-Filho" "autores" => array:4 [ 0 => array:2 [ "nombre" => "Maria Carolina Andrade" "apellidos" => "Maia" ] 1 => array:2 [ "nombre" => "Carolina Santos Souza" "apellidos" => "Tavares" ] 2 => array:2 [ "nombre" => "Cliomar Alves dos" "apellidos" => "Santos" ] 3 => array:2 [ "nombre" => "Paulo Ricardo" "apellidos" => "Martins-Filho" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2529993X2200243X?idApp=UINPBA00004N" "url" => "/2529993X/0000004000000010/v2_202212170724/S2529993X2200243X/v2_202212170724/en/main.assets" ] "en" => array:15 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Scientific letter</span>" "titulo" => "Use of the “FilmArray® blood culture identification 2” panel in a case of endophthalmitis due to <span class="elsevierStyleItalic">Candida parapsilosis</span>" "tieneTextoCompleto" => true "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "587" "paginaFinal" => "588" ] ] "autores" => array:1 [ 0 => array:4 [ "autoresLista" => "Domingo Fernández-Vecilla, Iris Sharon Pérez-Ramos, Cristina Aspichueta-Vivanco, Miren Josebe Unzaga Barañano" "autores" => array:4 [ 0 => array:4 [ "nombre" => "Domingo" "apellidos" => "Fernández-Vecilla" "email" => array:1 [ 0 => "domingofvec@gmail.com" ] "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">*</span>" "identificador" => "cor0005" ] ] ] 1 => array:2 [ "nombre" => "Iris Sharon" "apellidos" => "Pérez-Ramos" ] 2 => array:2 [ "nombre" => "Cristina" "apellidos" => "Aspichueta-Vivanco" ] 3 => array:2 [ "nombre" => "Miren Josebe" "apellidos" => "Unzaga Barañano" ] ] "afiliaciones" => array:1 [ 0 => array:2 [ "entidad" => "Hospital Universitario de Basurto, Bilbao (Vizcaya), Spain" "identificador" => "aff0005" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor0005" "etiqueta" => "⁎" "correspondencia" => "Corresponding author." ] ] ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Uso del panel «FilmArray® blood culture identification 2» en un caso de endoftalmitis causado por <span class="elsevierStyleItalic">Candida parapsilosis</span>" ] ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:8 [ "identificador" => "fig0005" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 478 "Ancho" => 955 "Tamanyo" => 56563 ] ] "detalles" => array:1 [ 0 => array:3 [ "identificador" => "at0005" "detalle" => "Figure " "rol" => "short" ] ] "descripcion" => array:1 [ "en" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">In the left eyeball, a linear image of V-shaped morphology fixed to the optic disc is visualised, suggestive of complete retinal detachment. In an internal location to the retina, other linear images are visualised, also fixed to the optic disc, suggestive of residual membranes of endophthalmitis.</p>" ] ] ] "textoCompleto" => "<span class="elsevierStyleSections"><p id="par0005" class="elsevierStylePara elsevierViewall">The FilmArray® (BioFire Diagnostics, Salt Lake City, UT, USA) system is a multiplex PCR that integrates sample preparation, amplification, detection and analysis. One of the available panels is the "Blood Culture Identification 2 (BCID2)", which allows for searching for up to 43 different targets associated with bacteraemia, including five antimicrobial resistance genes, directly from positive blood cultures. The objective of this text is to present our experience about the use of this PCR technique to help in the aetiological and therapeutic orientation in a case of endophthalmitis caused by <span class="elsevierStyleItalic">Candida parapsilosis (C. parapsilosis)</span>.</p><p id="par0010" class="elsevierStylePara elsevierViewall">We present the case of a 79-year-old man on anticoagulation and antiplatelet therapy with diabetes mellitus and hypertension, who underwent cataract surgery by phacoemulsification and intraocular lens implant without incident. Six months later, the patient has onset of pain, photophobia, and a slight decrease in visual acuity in that same eye. Upon examination, positive Tyndall effect, mild capsular phimosis and fibrosis, and whitish deposits are described. An ocular ultrasound is performed revealing data regarding complete retinal detachment and linear floaters similarly fixed to the optic disc suggestive of membranes residual to endophthalmitis (<a class="elsevierStyleCrossRef" href="#fig0005">Fig. 1</a>). A sample of vitreous humor (not diluted) is taken prior to infusion of saline solution, and subsequently a pars plana vitrectomy is performed. Samples are sent to the microbiology laboratory: vitreous and aqueous humor, as well as vitrectomy <span class="elsevierStyleItalic">cassette</span> and intraocular lens capsular bag complex. Intravitreal ceftazidime and vancomycin (1,000 mg and 500 mg diluted in 50 ml, respectively) are administered.</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia><p id="par0015" class="elsevierStylePara elsevierViewall">Upon arrival at the microbiology laboratory, the diluted aqueous and vitreous humor samples are processed by centrifugation (5 min at 3,000 rpm) and all but the last 0.5 ml of the supernatant is transferred to another tube. The sediment is resuspended in this 0.5 ml for Gram staining and culture inoculation. The samples are inoculated on chocolate agar (Becton Dickinson, Franklin Lakes, NJ, USA), Trypticase Soy Agar with 5% Sheep Blood (Becton Dickinson, Franklin Lakes, NJ, USA) and Sabouraud agar with chloramphenicol (Becton Dickinson, Franklin Lakes, NJ, USA), as well as in thioglycolate broth enrichment medium. Yeasts are observed in the Gram stain. At this point, 200 µl of the remaining supernatant is removed from the centrifuged vitreous humor for the performance of the FilmArray®BCID2 Panel, through which <span class="elsevierStyleItalic">C. parapsilosis</span> identified. After this result, the patient is taken to the operating room that same day for intravitreal injection of voriconazole (200 mg diluted in 20 ml). At 24 h, white colonies are observed in cultures of vitreous humor, aqueous humor, vitrectomy <span class="elsevierStyleItalic">cassette</span> and intraocular lens capsular bag complex, later being identified as <span class="elsevierStyleItalic">C. parapsilosis</span> by MALDI-TOF (matrix-assisted laser desorption/ionisation mass spectrometry, Bruker™). The patient is discharged from the hospital with oral fluconazole 100 mg/24 h and corticosteroids 2.5 mg/48 h, as well as tobramycin/corticosteroid 1 mg/mL and voriconazole 10 mg/mL/4 h eye drops.</p><p id="par0020" class="elsevierStylePara elsevierViewall">The FilmArray® BCID2 panel multiplex PCR system has been extensively tested, with numerous studies demonstrating high sensitivity and specificity in positive blood culture samples.<a class="elsevierStyleCrossRefs" href="#bib0005"><span class="elsevierStyleSup">1,2</span></a> Its applicability to sterile samples other than blood cultures has also been studied for therapeutic guidance in both the adult and paediatric population.<a class="elsevierStyleCrossRefs" href="#bib0015"><span class="elsevierStyleSup">3–6</span></a> Even in the context of septic arthritis or pleural empyema, where the consistency of the sample and the high leukocyte concentration could inhibit this test, this technique could be a helpful complementary diagnostic tool.<a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">7</span></a></p><p id="par0025" class="elsevierStylePara elsevierViewall">It is rare that the FilmArray® multiplex PCR system yields false negatives, but Gonzalez-Donapetry et al. presented a representative example.<a class="elsevierStyleCrossRef" href="#bib0040"><span class="elsevierStyleSup">8</span></a> Following their recommendations, the <span class="elsevierStyleItalic">melting</span> curves and internal controls must be carefully reviewed because at times there may be alterations, the meaning of which could differ depending on the clinical context.</p><p id="par0030" class="elsevierStylePara elsevierViewall">The FilmArray® BCID2 system has demonstrated some utility in sterile samples other than those indicated by the manufacturer, and it is a tool to consider when it is necessary to establish early antibiotic treatment in patients and complex situations, such as the case of endophthalmitis that we present. Despite this, the limitations of the technique in this type of sample must be taken into account, carrying out a prior validation, and further prospective studies that add more evidence to what has been observed up until now.</p><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0005">Author contributions</span><p id="par0035" class="elsevierStylePara elsevierViewall"><ul class="elsevierStyleList" id="lis0005"><li class="elsevierStyleListItem" id="lsti0005"><span class="elsevierStyleLabel">•</span><p id="par0040" class="elsevierStylePara elsevierViewall">Domingo Fernández Vecilla: drafted the scientific text.</p></li><li class="elsevierStyleListItem" id="lsti0010"><span class="elsevierStyleLabel">•</span><p id="par0045" class="elsevierStylePara elsevierViewall">Iris Sharon Pérez Ramos: helped draft the clinical case.</p></li><li class="elsevierStyleListItem" id="lsti0015"><span class="elsevierStyleLabel">•</span><p id="par0050" class="elsevierStylePara elsevierViewall">Cristina Aspichueta Vivanco: reviewed the case and helped modify it.</p></li><li class="elsevierStyleListItem" id="lsti0020"><span class="elsevierStyleLabel">•</span><p id="par0055" class="elsevierStylePara elsevierViewall">Miren Josebe Unzaga Barañano: reviewed the case and helped modify it.</p></li></ul></p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Funding</span><p id="par0060" class="elsevierStylePara elsevierViewall">This text has not received funding.</p></span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Conflicts of interest</span><p id="par0065" class="elsevierStylePara elsevierViewall">The authors declare that they have no conflicts of interest.</p></span></span>" "textoCompletoSecciones" => array:1 [ "secciones" => array:4 [ 0 => array:2 [ "identificador" => "sec0005" "titulo" => "Author contributions" ] 1 => array:2 [ "identificador" => "sec0010" "titulo" => "Funding" ] 2 => array:2 [ "identificador" => "sec0015" "titulo" => "Conflicts of interest" ] 3 => array:1 [ "titulo" => "References" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "NotaPie" => array:1 [ 0 => array:2 [ "etiqueta" => "☆" "nota" => "<p class="elsevierStyleNotepara" id="npar0005">Please cite this article as: Fernández-Vecilla D, Pérez-Ramos IS, Aspichueta-Vivanco C, Unzaga-Barañano MJ. Uso del panel «FilmArray® blood culture identification 2» en un caso de endoftalmitis causado por <span class="elsevierStyleItalic">Candida parapsilosis</span>, Enferm Infecc Microbiol Clin. 2022;40:587–588.</p>" ] ] "multimedia" => array:1 [ 0 => array:8 [ "identificador" => "fig0005" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 478 "Ancho" => 955 "Tamanyo" => 56563 ] ] "detalles" => array:1 [ 0 => array:3 [ "identificador" => "at0005" "detalle" => "Figure " "rol" => "short" ] ] "descripcion" => array:1 [ "en" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">In the left eyeball, a linear image of V-shaped morphology fixed to the optic disc is visualised, suggestive of complete retinal detachment. In an internal location to the retina, other linear images are visualised, also fixed to the optic disc, suggestive of residual membranes of endophthalmitis.</p>" ] ] ] "bibliografia" => array:2 [ "titulo" => "References" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "bibs0005" "bibliografiaReferencia" => array:8 [ 0 => array:3 [ "identificador" => "bib0005" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Valoración de una técnica de PCR-múltiple en el diagnóstico rápido de la bacteriemia" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "M.F. López-Fabal" 1 => "J.L. Gómez-Garcés" 2 => "M. López Lomba" 3 => "M. Ruiz Bastián" ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.5858/arpa.2012-0651-OA" "Revista" => array:6 [ "tituloSerie" => "Rev Esp Quimioter." 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Year/Month | Html | Total | |
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2024 October | 23 | 7 | 30 |
2024 September | 82 | 9 | 91 |
2024 August | 44 | 3 | 47 |
2024 July | 22 | 13 | 35 |
2024 June | 27 | 9 | 36 |
2024 May | 11 | 4 | 15 |
2024 April | 26 | 3 | 29 |
2024 March | 12 | 2 | 14 |
2024 February | 8 | 1 | 9 |
2024 January | 17 | 3 | 20 |
2023 December | 12 | 0 | 12 |
2023 November | 12 | 5 | 17 |
2023 October | 11 | 1 | 12 |
2023 September | 7 | 0 | 7 |
2023 August | 12 | 1 | 13 |
2023 July | 2 | 4 | 6 |
2023 June | 1 | 4 | 5 |
2023 April | 1 | 0 | 1 |
2023 January | 4 | 0 | 4 |
2022 December | 7 | 0 | 7 |