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Journal Information
Vol. 48. Issue 3.
Pages 295-305 (May - June 2020)
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Vol. 48. Issue 3.
Pages 295-305 (May - June 2020)
Original Article
DOI: 10.1016/j.aller.2019.12.009
Reference values of leukocyte and lymphocytes populations in umbilical cord and capillary blood in healthy Mexican newborns
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S. Scheffer-Mendozaa, S.E. Espinosa-Padillab, G. López-Herrerac, F. Mujica-Guzmánc, M.G. López-Padillad, L. Berrón-Ruizb,
Corresponding author
lberronruiz@yahoo.com.mx

Corresponding author at: Unidad de Investigación en Inmunodeficiencias, Instituto Nacional de Pediatria, SSa, Insurgentes Sur # 3700-C, Colonia Insurgentes Cuicuilco, Coyoacán, C.P. 04530, Ciudad de México, Mexico.
a Servicio de Inmunología y Alergia, Instituto Nacional de Pediatría, SSa, Ciudad de México, Mexico
b Unidad de Investigación en Inmunodeficiencias, Instituto Nacional de Pediatría, SSa, Ciudad de México, Mexico
c Laboratorio de Hematología, Instituto Nacional de Pediatría, SSa, Ciudad de México, Mexico
d Unidad Tocoquirúgica, Instituto Nacional de Enfermedades Respiratorias “Dr. Manuel Gea González”, SSa, Ciudad de México, Mexico
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Tables (2)
Table 1. Absolute numbers (μl of blood) and percentages (%) of total leukocytes and subpopulations of lymphocytes in cord blood and capillary blood of healthy newborns (n=50).
Table 2. Absolute numbers (μl of blood) and percentages (%) of total leukocytes and subpopulations of lymphocytes in cord blood and capillary blood of male (n=28) and female (n=21) healthy newborns.
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Abstract
Introduction

In newborns, dramatic changes occur in the blood and bone marrow during the first hours; there are rapid fluctuations in the quantities of leukocytes populations. In this work, we investigated leukocytes subsets counts in two types of blood samples (cord blood and capillary blood) extracted from healthy newborns.

Methods

Blood samples from Mexican neonates were collected by Instituto Nacional de Pediatría with written informed consent. For all samples we determined leukocytes populations; neutrophils, monocytes, total lymphocytes, and populations: T CD3+ cells, TCD4+ cells, T CD8+ cells, B CD19+ cells and NK CD16+56 cells by flow cytometry. We used the Mann–Whitney U test to compare leukocytes of cord and capillary blood; also to analyze the differences between gender and we obtained reference values of the cord and capillary blood in neonates.

Results

We observed higher absolute counts and frequencies of total lymphocyte in capillary blood compared with cord blood. In absolute numbers, the capillary blood showed significant differences in neutrophils, monocytes, lymphocytes, T CD3+ cells, T CD4+ cells, T CD8+ cells, B CD19+ cells, and NK cells; no significant differences were observed between genders.

Discussion

Our data contribute to newborn Mexican reference values for all these populations of leukocytes. We found that the dispersal range differs between the two types of blood, suggesting a different fate in the immune response. Immunophenotyping of the blood cell population to identify these cells is an essential tool in the diagnosis and follow-up of neonates with immunodeficiencies and other immune disorders.

Keywords:
Capillary blood
Umbilical cord blood
Leukocytes
Newborn
Reference value
Lymphocyte populations

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