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Inicio Allergologia et Immunopathologia Reduced Akkermansia muciniphila and Faecalibacterium prausnitzii levels in the g...
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Vol. 47. Issue 4.
Pages 365-371 (July - August 2019)
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Vol. 47. Issue 4.
Pages 365-371 (July - August 2019)
Original Article
DOI: 10.1016/j.aller.2018.12.009
Reduced Akkermansia muciniphila and Faecalibacterium prausnitzii levels in the gut microbiota of children with allergic asthma
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M. Demircia, H.B. Tokmanb, H.K. Uysalc, S. Demiryasd, A. Karakullukcue, S. Saribasb, H. Cokugrasf, B.S. Kocazeybekb,
Corresponding author
bzeybek@istanbul.edu.tr

Corresponding author.
a Department of Medical Microbiology, Medical Faculty, Beykent University, Istanbul 34580, Turkey
b Department of Medical Microbiology, Cerrahpaşa Medical Faculty, Istanbul University-Cerrahpaşa, Istanbul 34320, Turkey
c Department of Medical Microbiology, Istanbul Medical Faculty, Istanbul University, Istanbul 34300, Turkey
d Department of General Surgery, Cerrahpaşa Medical Faculty, Istanbul University-Cerrahpaşa, Istanbul 34320, Turkey
e Vocational School of Health Services, Gümüşhane University, Gümüşhane 29100, Turkey
f Department of Pediatric Infectious Diseases, Clinical Immunology and Allergy, Cerrahpaşa Medical Faculty, Istanbul University-Cerrahpaşa, Istanbul 34200, Turkey
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Table 1. Demographical features and some laboratory parameters of patient and control groups.
Table 2. Distribution of A. muciniphila and F. prausnitzii in asthma patient and healthy control groups (log10copy/μg).
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Abstract
Introduction and objectives

The amounts of Akkermansia muciniphila and Faecalibacterium prausnitzii in gut microbiota are reduced in patients with allergic diseases compared to healthy controls. We aimed to quantify levels of A. muciniphila and F. prausnitzii amounts using real-time quantitative PCR (qPCR) in the gut microbiota of children with allergic asthma and in healthy controls.

Materials and methods

In total, 92 children between the ages of three and eight who were diagnosed with asthma and 88 healthy children were included in the study and bacterial DNA was isolated from the stool samples using the stool DNA isolation Kit. qPCR assays were studied with the microbial DNA qPCR Kit for A. muciniphila and microbial DNA qPCR Kit for F. prausnitzii.

Results

Both bacterial species showed a reduction in the patient group compared to healthy controls. A. muciniphila and F. prausnitzii were found to be 5.45±0.004, 6.74±0.01 and 5.71±0.002, 7.28±0.009 in the stool samples of the asthma and healthy control groups, respectively.

Conclusions

F. prausnitzii and A. muciniphila may have induced anti-inflammatory cytokine IL-10 and prevented the secretion of pro-inflammatory cytokines like IL-12. These findings suggest that A. muciniphila and F. prausnitzii may suppress inflammation through its secreted metabolites.

Keywords:
Asthmatic children
Akkermansia muciniphila
Faecalibacterium prausnitzii
Hygiene hypothesis
qPCR

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