Buscar en
Allergologia et Immunopathologia
Toda la web
Inicio Allergologia et Immunopathologia Identification of polcalcin as a novel allergen of Amaranthus retroflexus pollen
Journal Information
Vol. 47. Issue 4.
Pages 357-364 (July - August 2019)
Download PDF
More article options
Vol. 47. Issue 4.
Pages 357-364 (July - August 2019)
Original Article
DOI: 10.1016/j.aller.2018.12.006
Identification of polcalcin as a novel allergen of Amaranthus retroflexus pollen
M. Vakili Moghaddama,b, M. Fallahpourc, M. Mohammadid, F.S. Rasi Varaeea, K. Mokhtariane, M. Khoshmirsafaa,g, R. Jafarif, N. Shirzadb, R. Falaka,g,
Corresponding author
a Immunology Research Center, Iran University of Medical Sciences, Tehran, Iran
b Department of Immunology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran
c Department of Allergy and Clinical Immunology, Iran University of Medical Sciences, Tehran, Iran
d Persian Gulf Marine Biotechnology Research Center, Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
e Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
f Department of Immunology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
g Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
This item has received
Article information
Full Text
Download PDF
Figures (3)
Show moreShow less
Tables (2)
Table 1. Clinical characteristics, IgE-reactivity and SPT responses of the A. retroflexus allergic patients.
Table 2. Comparison of specific IgE-reactivity of A. retroflexus and recombinant Ama r 3 with different cut-off points of absorbance (450nm) using ELISA test.
Show moreShow less

Amaranthus retroflexus (Redroot Pigweed) is one of the main sources of allergenic pollens in temperate areas. Polcalcin is a well-known panallergen involved in cross-reactivity between different plants. The aim of this study was the molecular cloning and expression of polcalcin, as well as evaluating its IgE-reactivity with A. retroflexus sensitive patients’ sera.


Allergenic extract was prepared from A. retroflexus pollen and the IgE-reactivity profile was determined by ELISA and immunoblotting using sera from twenty A. retroflexus sensitive patients. Polcalcin-coding sequence was amplified by conventional PCR method and the product was inserted into pET-21b(+) vector. The recombinant protein was expressed in E. coli BL21 and purified by metal affinity chromatography. The IgE-binding capability of the recombinant protein was analyzed by ELISA and immunoblotting assays, and compared with crude extract.


Of 20 skin prick test positive patients, 17 patients were positive in IgE-specific ELISA. Western blotting confirmed that approximately 53% of ELISA positive patients reacted with 10kDa protein in crude extract. The A. retroflexus polcalcin gene, encoding to 80 amino acid residues was cloned and expressed as a soluble protein and designated as Ama r 3. The recombinant polcalcin showed rather identical IgE-reactivity in ELISA and western blotting with 10kDa protein in crude extract. These results were confirmed by inhibition methods, too.


The recombinant form of A. retroflexus polcalcin (Ama r 3) could be easily produced in E. coli in a soluble form and shows rather similar IgE-reactivity with its natural counterpart.

Allergen characterization
Amaranthus retroflexus


These are the options to access the full texts of the publication Allergologia et Immunopathologia

If you already have your login data, please click here .

If you have forgotten your password you can you can recover it by clicking here and selecting the option “I have forgotten my password”
Subscribe to

Allergologia et Immunopathologia

Comprar acceso al artículo

Comprando el artículo el PDF del mismo podrá ser descargado

Precio 22,50 €

Comprar ahora
Phone for subscriptions and reporting of errors
From Monday to Friday from 9 a.m. to 6 p.m. (GMT + 1) except for the months of July and August which will be from 9 a.m. to 3 p.m.
Calls from Spain
932 415 960
Calls from outside Spain
+34 932 415 960
Article options
es en pt

¿Es usted profesional sanitario apto para prescribir o dispensar medicamentos?

Are you a health professional able to prescribe or dispense drugs?

Você é um profissional de saúde habilitado a prescrever ou dispensar medicamentos

es en pt
Política de cookies Cookies policy Política de cookies
Utilizamos cookies propias y de terceros para mejorar nuestros servicios y mostrarle publicidad relacionada con sus preferencias mediante el análisis de sus hábitos de navegación. Si continua navegando, consideramos que acepta su uso. Puede cambiar la configuración u obtener más información aquí. To improve our services and products, we use "cookies" (own or third parties authorized) to show advertising related to client preferences through the analyses of navigation customer behavior. Continuing navigation will be considered as acceptance of this use. You can change the settings or obtain more information by clicking here. Utilizamos cookies próprios e de terceiros para melhorar nossos serviços e mostrar publicidade relacionada às suas preferências, analisando seus hábitos de navegação. Se continuar a navegar, consideramos que aceita o seu uso. Você pode alterar a configuração ou obter mais informações aqui.