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Inicio Allergologia et Immunopathologia Effect of acid and in vitro digestion on conformation and IgE-binding capacity o...
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Vol. 48. Issue 1.
Pages 26-33 (January - February 2020)
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Vol. 48. Issue 1.
Pages 26-33 (January - February 2020)
Original Article
DOI: 10.1016/j.aller.2019.08.001
Effect of acid and in vitro digestion on conformation and IgE-binding capacity of major oyster allergen Cra g 1 (tropomyosin)
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Jiangtao Zhang1, Wenying Liu1, Lei Fang, Ruizeng Gu, Jun Lu
Corresponding author
Johnljsmith@163.com

Corresponding authors at: China National Research Institute of Food & Fermentation Industries, No.24, Jiuxianqiao Middle Road, Chaoyang District, Beijing, China.
, Guoming Li
Corresponding author
297756628@qq.com

Corresponding authors at: China National Research Institute of Food & Fermentation Industries, No.24, Jiuxianqiao Middle Road, Chaoyang District, Beijing, China.
Beijing Engineering Research Center of Protein & Functional Peptides, China National Research Institute of Food and Fermentation Industries, Beijing 100015, PR China
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Abstract
Introduction and Objectives

The production and consumption of oysters is increasing annually because it can provide essential nutrients and benefit for human health, leading to frequent occurrence of severe allergic reactions observed in sensitized individuals. The aim of the present study was to investigate the effects of acid and protease treatment on the conformation and IgE-binding capacity of recombinant Crassostrea gigas tropomyosin (Cra g 1).

Results

Under acidic conditions, Cra g 1 did not undergo degradation, however, the changes obvious in the intensity of CD signal and ANS-binding fluorescence were observed, which was associated with a decrease in antibody reactivity. In simulated gastrointestinal fluid (SGF) and simulated intestinal fluid (SIF) digestion system, acid-treated Cra g 1 was relatively resistant to digestion, but the degradative patterns were very different. Moreover, owing to alterations of secondary structure and hydrophobic surface of the protein during digestive processing, antigenicity of acid-induced Cra g 1 reduced in SGF while it increased significantly in SIF.

Conclusion

To our knowledge, this is the first study reporting that antigenicity of acid-treated oyster tropomyosin increased after SIF digestion. These results revealed that treatment with acid and pepsin, rather than trypsin, was an effective way of reducing IgE-binding capacity of tropomyosin from oyster.

Keywords:
Cra g1
Acid
SGF
SIF
Conformation
IgE-binding

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