Regístrese
Buscar en
Allergologia et Immunopathologia
Toda la web
Inicio Allergologia et Immunopathologia Staining of antinuclear antibodies and antibodies against removable nuclear anti...
Journal Information
Vol. 48. Issue 1.
Pages 18-25 (January - February 2020)
Share
Share
Download PDF
More article options
ePub
Vol. 48. Issue 1.
Pages 18-25 (January - February 2020)
Original Article
DOI: 10.1016/j.aller.2019.07.002
Staining of antinuclear antibodies and antibodies against removable nuclear antigens in connective tissue diseases
Visits
6
José Enrique Oliva Menachoa,b,⿿
Corresponding author
jose.enrique.oliva@hotmail.com
jose.oliva.m@upch.pe

Corresponding author at: Jr. José María Morellos 207 Dpt.402, San Miguel, Lima, Peru.
, Jorge Luis Arroyo-Acevedoc,d, José Arturo Oliva-Candelae, Marco Antonio García-Hjarlesf, Lester Domínguez-Huarcayag
a Faculty of Medicine, Cayetano Heredia Peruvian University, Lima, Peru
b Postgraduate unit of the Faculty of Medicine of the National Major University of San Marcos, Arzobispo Loayza National Hospital, Lima, Peru
c Institute of Clinical Research, Faculty of Medicine, National University of San Marcos, Lima, Peru
d Laboratory of Experimental Pharmacology, Faculty of Medicine, National University of San Marcos, Lima, Peru
e Arzobispo Loayza National Hospital, Lima, Peru
f Faculty of Sciences and philosophy, Faculty of Medicine, Cayetano Heredia Peruvian University, Lima, Peru
g Institute of Clinical Research, Faculty of Medicine, National University of San Marcos. Faculty of Medicine of the University Norbert Wiener, Lima, Peru
Article information
Abstract
Full Text
Bibliography
Download PDF
Statistics
Tables (5)
Table 1. Relationship between the homogeneous pattern and anti-dsDNA in patients with connective tissue disease attended at the national Hospital Archbishop Loayza between January and June 2017.
Table 2. Relationship between the homogeneous pattern and anti-Ro 52 in patients with connective tissue disease attended at the national Hospital Archbishop Loayza between January and June 2017.
Table 3. Relationship between the mottled pattern and anti-Ro 52 in patients with connective tissue disease attended at the national Hospital Archbishop Loayza between January and June 2017.
Table 4. Relationship between the mottled pattern and the anti-SSB in patients with connective tissue disease attended at the national Hospital Archbishop Loayza between January and June 2017.
Table 5. Relationship between the centromeric pattern and the anti-CENP B in patients with connective tissue disease attended at the national Hospital Archbishop Loayza between January and June 2017.
Show moreShow less
Abstract
Introduction and objectives

Connective tissue diseases are inflammatory, autoimmune diseases and threaten quality of life. To determine the relationship between staining patterns of antinuclear antibodies and antibodies against extractable nuclear antigens in patients with connective tissue disease.

Materials and methods

Observational, basic, analytical and transversal study. Study conducted in the Immunology Service of the Arzobispo Loayza National Hospital between January 2017 and June 2017. We analyzed 291 samples of patients with CTD and for the detection of anti-nuclear antibody staining patterns, the immunological kit and observation with microscope of at 40X Immunofluorescence and for the detection of the antibodies against extractable nuclear antigens. The Immunoblot method was employed. Statistical analyses were carried out with the statistical package SPSS version 21 for Windows. We used the Pearson Chi-square test for the categorical variables, a value of p<0.05 was considered significant.

Results

There was a significant relationship p<0.05 of the homogeneous pattern, the mottled pattern with Anti-histones (p=0.000), Anti-nucleosomes (p=0.000), Anti-Ro 52 (p=0.000), Anti-SSA (p=0.001), Anti-SSB (p=0.003), Anti-dsDNA (p=0.000) with the Pearson Chi-square test. There was a significant relationship of p<0.05 of the centromeric pattern with Anti-Cenp B (p=0.000) with Fisher⿿s exact statistic.

Conclusions

There was a significant relationship between the anti-nuclear antibody staining patterns and the antibodies to the core extractable antigens in patients with systemic lupus erythematosus, Sjögren⿿s syndrome, Calcinosis, Raynaud⿿s phenomenon, esophageal Dysmotility, sclerodactyly and Telangiectasia (CREST), Scleroderma and Polymyositis.

Keywords:
Antibodies against extractable nuclear antigens
Anti-Nuclear antibody staining pattern
Connective tissue diseases
Immunoblot
Indirect immunofluorescence

Article

These are the options to access the full texts of the publication Allergologia et Immunopathologia
Subscriber
Subscriber

If you already have your login data, please click here .

If you have forgotten your password you can you can recover it by clicking here and selecting the option “I have forgotten my password”
Subscribe
Subscribe to

Allergologia et Immunopathologia

Comprar
Comprar acceso al artículo

Comprando el artículo el PDF del mismo podrá ser descargado

Precio 22,50 €

Comprar ahora
Contact
Phone for subscriptions and reporting of errors
From Monday to Friday from 9 a.m. to 6 p.m. (GMT + 1) except for the months of July and August which will be from 9 a.m. to 3 p.m.
Calls from Spain
932 415 960
Calls from outside Spain
+34 932 415 960
E-mail
Article options
Tools
es en pt

¿Es usted profesional sanitario apto para prescribir o dispensar medicamentos?

Are you a health professional able to prescribe or dispense drugs?

Você é um profissional de saúde habilitado a prescrever ou dispensar medicamentos

es en pt
Política de cookies Cookies policy Política de cookies
Utilizamos cookies propias y de terceros para mejorar nuestros servicios y mostrarle publicidad relacionada con sus preferencias mediante el análisis de sus hábitos de navegación. Si continua navegando, consideramos que acepta su uso. Puede cambiar la configuración u obtener más información aquí. To improve our services and products, we use "cookies" (own or third parties authorized) to show advertising related to client preferences through the analyses of navigation customer behavior. Continuing navigation will be considered as acceptance of this use. You can change the settings or obtain more information by clicking here. Utilizamos cookies próprios e de terceiros para melhorar nossos serviços e mostrar publicidade relacionada às suas preferências, analisando seus hábitos de navegação. Se continuar a navegar, consideramos que aceita o seu uso. Você pode alterar a configuração ou obter mais informações aqui.