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Archivos de la Sociedad Española de Oftalmología (English Edition) 30-Gauge needle Descemet membrane endothelial keratoplasty graft obtention: A no...
Journal Information
Vol. 100. Issue 11.
Pages 742-745 (November 2025)
Vol. 100. Issue 11.
Pages 742-745 (November 2025)
Short communication
30-Gauge needle Descemet membrane endothelial keratoplasty graft obtention: A novel, safer and cheap technique
Obtención del injerto para queratoplastia endotelial de la membrana de Descemet con aguja de calibre 30 Gauge: una técnica novedosa, segura y económica
P. Pérez-Garcíaa,1,
Corresponding author
perezgarciapilar.ppg@gmail.com

Corresponding author.
, J.A. Gegúndez-Fernándeza,b,1, M. Ariño-Gutiérreza, M. Molero-Senosiaína, B. Burgos-Blascoa, D. Díaz-Vallea,b
a Servicio de Oftalmología, Hospital Clínico San Carlos, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid, Spain
b Departamento de Inmunología, Oftalmología y ORL, Facultad de Medicina, Universidad Complutense de Madrid, Madrid, Spain
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Abstract

This work aims to present a novel technique for obtaining grafts for Descemet Membrane Endothelial Keratoplasty (DMEK) safely and efficiently, thanks to its fast learning curve and reduced risk of graft tears. The technique involves the peripheral dissection of the endothelium starting with a superficial incision made using a 30-Gauge needle, 1 mm inside Schwalbe's line across 360º of the corneal periphery. Trypan Blue is then applied to visualize the dissection boundary, and a DMEK Sinskey hook is used to lift 1 mm of the peripheral graft along the entire circumference. The technique continues with the separation of Descemet's membrane from the stroma, as is commonly performed. This technique reduces costs by employing a standard needle and achieves low failure rates compared to other techniques.

Keywords:
Descemet membrane endothelial keratoplasty
DMEK
Graft preparation
Preparation technique
Needle
Resumen

El objetivo de este trabajo es presentar esta novedosa técnica para obtener injertos para queratoplastia endotelial de membrana de Descemet (DMEK) de manera segura y eficiente, gracias a su rápida curva de aprendizaje y menor riesgo de desgarros en el injerto. La técnica consiste en la disección periférica del endotelio a partir de una incisión superficial realizada con una aguja de calibre 30 G a 1 mm de la línea de Schwalbe en los 360º de la periferia corneal. A continuación, se aplica azul tripán para visualizar el límite de la disección y mediante un Simskey de DMEK se levanta 1 mm del injerto periférico en toda la circunferencia. La técnica continúa con el pelado de la membrana de Descemet, como es habitual. Esta técnica reduce los costes al utilizar una aguja estándar y logra bajas tasas de fallo en comparación con otras técnicas.

Palabras clave:
Queratoplastia endotelial de Membrana de Descemet
DMEK
Injerto
Preparación
Aguja

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