Abstracts of the 2025 Annual Meeting of the ALEH
More infoMoringa oleifera (moringa) extracts have hepatoprotective, antioxidant, and anticancer effects. This study aimed to determine the effect of moringa extract on an in vivo model of liver cancer.
Materials and MethodsMoringa leaves powder was simmered in water at 80ºC and its antioxidant capacity was determined using ABTS and DPPH methods. Male Wistar rats were divided in groups: control (Ctl), damage (Dmg), aqueous extract (Leaves-AqE), and 4) damage and Leaves-AqE (Dmg+Leaves-AqE). Leaves-AqE (300mg/kg i.g) was administered daily for two weeks and three times a week during 18 weeks. Since the second week, damage was induced by weekly administration of DEN and 2-AAF for 18 weeks. Serum biochemistry and gene expression were analyzed. Statistical analysis was performed using GraphPad Prism 10 (p<0.05). UDG Committees approved the protocol (Code CI-01720). The authors declare no conflicts of interest.
ResultsLeaves-AqE had an antioxidant capacity slightly more than 70% in vitro. The administration of the extract led to a decrease in liver tumor development and prevented mortality by the damage treatment. Leaves-AqE did not have significant effects on serum hepatic function markers. Interestingly, CAT and SOD were low in the Dmg+Leaves-AqE group. In addition, TGFB1 showed a tendency to decrease in the Dmg+Leaves-AqE group compared to Dmg group.
ConclusionsThe Leaves-AqE extract has an important antioxidant inhibitory capacity in vitro. This capacity may explain why supplementation with this extract increased survival as well as the tendency to reduce the expression of TGFB1 induced by chemical damage.
