Buscar en
Porto Biomedical Journal
Toda la web
Inicio Porto Biomedical Journal Examination of antiproliferative effects of the horseradish extracts
Journal Information
Vol. 2. Issue 5.
Pages 213 (September - October 2017)
Share
Share
Download PDF
More article options
Vol. 2. Issue 5.
Pages 213 (September - October 2017)
PS075
Open Access
Examination of antiproliferative effects of the horseradish extracts
Visits
1571
L. Đurić1,
Corresponding author
djuriclarisa@gmail.com

Corresponding author.
, D. Četojević-Simin2, M. Milanović1
1 University of Novi Sad, Faculty of Medicine, Department of Pharmacy, Novi Sad, Serbia
2 University of Novi Sad, Faculty of Medicine, Experimental Oncology Department, Oncology Institute of Vojvodina, Sremska Kamenica, Serbia
This item has received

Under a Creative Commons license
Article information
Full Text

Aim: The aim of the study was to investigate in vitro the antiproliferative effects of the horseradish juice and pulp using different solvents for the extraction.

Introduction: Horseradish (Armoracia rusticana, Brassicaceae) is a perennial herbal plant, which is widely used in human nutrition, as well as in a traditional medicine. Horseradish is a rich source of bioactive compounds such as isothiocyanates, that have proved to be significant antitumor agents.

Methods: Samples were prepared by the Kupchak extraction method, and the antiproliferative effects of the horseradish juice and pulp extracts were examined on the human tumor cell line MDA-MB-231 (ER−, human breast adenocarcinoma). Cell growth was determined by measuring the total protein by colorimetric sulforhodamine B assay. The obtained results (expressed as mean±SD) were analyzed by Tukey HSD test and the differences were considered statistically significant at p<0.05.

Results: According to the IC50 parameter (the concentration that inhibited the cell growth by 50%), as an important indicator of the antiproliferative effects, the most pronounced antitumor activity was observed for chloroform juice extract (IC50=5.52±1.47μg/ml). In addition, highly potent was chloroform pulp extract (IC50=19.44±3.82μg/ml), as well as the dichloromethane juice (IC50=26.50±4.15μg/ml) and pulp (IC50=26, 01±2.45μg/ml) extracts. On the other hand, significantly lower in vitro antitumor effect was noticed for the butanol pulp extract (IC50=114.52±0.28μg/ml). IC50 vales for butanol juice extract, as well as water juice and pulp extracts were higher than 500μg/ml.

Conclusion: The obtained results suggest that A. rusticana is as a significant source of antitumor agents, especially liposoluble isothiocyanates and as such, it should be recommended for further use in a human nutrition and prevention of cancer.

Article options
Tools
es en pt

¿Es usted profesional sanitario apto para prescribir o dispensar medicamentos?

Are you a health professional able to prescribe or dispense drugs?

Você é um profissional de saúde habilitado a prescrever ou dispensar medicamentos