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Vol. 41. Issue 8.
Pages 504-510 (October 2017)
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Vol. 41. Issue 8.
Pages 504-510 (October 2017)
Original article
DOI: 10.1016/j.acuroe.2017.07.004
Is there room for luminal-basal urothelial cell population quantification?
¿Hay lugar para la cuantificación de la población de células uroteliales luminales basales?
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G.R. Passosa, J.A. Camargoa, K.L. Ferraria, G.C. Francha, A.E. Nowilla, L.O. Reisb,
Corresponding author
reisleo.l@gmail.com

Corresponding author.
a School of Medical Sciences, University of Campinas, UNICAMP, Campinas, São Paulo, Brazil
b Urologic Oncology Department, Pontifical Catholic University of Campinas PUC-Campinas, São Paulo, Brazil
Article information
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Abstract
Purpose

Three cell layers compose the urothelium: basal, intermediate and luminal (“umbrella cells”) and different diseases might arise from different cell populations. The aim of this study is to analyze the quantification ability of such cell populations by using four different protocols.

Methods

Twenty male rats (Wistar) were randomized in four groups of five animals: scraping, enzymatic 30, 45 and 60min. The cells were isolated, analyzed by flow cytometer and data processed by BD FACSDIVA™ software.

Results

The urothelium was separated in two cell populations that are different in size and complexity. The group that showed more efficiency in cells dissociation and cells separation was enzymatic protocol 45min.

Conclusions

Enzymatic protocol 45min was able to isolate urothelial cell populations and might be explored as potential prognostic tool, patient selection and therapeutic target in urothelial diseases. Future studies should validate the potential clinical application to the proposed rational of luminal-basal paradigm in the urothelial cancer as hope for individualized approach.

Keywords:
Luminal
Basal
Intermediate
Prognostic
Urothelium
Resumen
Propósito

Tres capas celulares componen el urotelio: basal, intermedio y luminal («células en paraguas») y diferentes enfermedades pueden surgir de diferentes poblaciones celulares. El objetivo de este estudio es analizar la capacidad de cuantificación de dichas poblaciones celulares utilizando 4 protocolos diferentes.

Métodos

Se aleatorizaron 20 ratas macho (Wistar) en 4 grupos de 5 animales: raspado, enzimático 30, 45 y 60minutos. Las células se aislaron, se analizaron mediante citómetro de flujo y se procesaron datos mediante el software BD FACSDIVA™.

Resultados

El urotelio se separó en 2 poblaciones celulares que son diferentes en tamaño y complejidad. El grupo que mostró más eficiencia en la disociación de células y la separación celular fue el protocolo enzimático de 45minutos.

Conclusiones

El protocolo enzimático de 45minutos fue capaz de aislar las poblaciones de células uroteliales, y podría ser explorado como herramienta pronóstica potencial, selección de pacientes y objetivo terapéutico en las enfermedades uroteliales. Estudios futuros deberían validar la aplicación clínica potencial al racional propuesto del paradigma luminal basal en el cáncer urotelial como esperanza para un abordaje individualizado.

Palabras clave:
Luminal
Basal
Intermedio
Pronóstico
Urotelio

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