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Original Article
DOI: 10.1016/j.aller.2019.12.003
Available online 11 April 2020
Identification of the major allergenic proteins from silkworm moth (Bombyx mori) involved in respiratory allergic diseases
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Laura M.L. Araujoa,
Corresponding author
laura.araujo80@gmail.com

Corresponding author.
, Claudemir Souzab, Nilson I.T. Zanchinb, Nelson A. Rosário Filhoa
a Programa de Pós-graduação em Saúde da Criança e do Adolescente, Universidade Federal do Paraná, Brazil
b Instituto Carlos Chagas – FIOCRUZ, Curitiba, Paraná, Brazil
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Table 1. Epidemiological, clinical and SPT sensitivity data from participants (n 36).
Abstract
Introduction and Objectives

Moths are a significant source of indoor and outdoor aeroallergens. High prevalence of IgE-mediated sensitization was demonstrated in a group of patients with allergic respiratory diseases. There are no studies on adult stage of these moth species allergens involved in allergic respiratory reactions - the aim of this study.

Material and Methods

36 participants were included in an experimental study, submitted to skin prick test with Bombyx mori wing extract and six other common allergens, as well as Western blot analysis with incubated nitrocellulose membrane impregnated with silkworm moth extract and human IgE-antibody. The participants were divided into 3 groups: 1) 21 allergic patients whose skin prick test was positive to Bombyx mori wing extract, 2) eight allergic patients whose skin prick test was positive to mite and negative to Bombyx mori extract 3) seven negative non-allergic subjects.

Results

Among the 21 participants from group 1, 19 serum samples reacted to Bombyx mori extract by Western blot. All of them reacted to a protein at 80 kDa and five other proteins (66, 50, 45, 37 and 30 kDa) were identified in more than 50% of the individuals tested, considered as major allergenic proteins. Sera from seven out of eight patients sensitized to house dust mite demonstrated IgE-reactivity to Bombyx mori extract by Western blot analysis. Serum samples from healthy participants did not react at all.

Conclusion

Six major reactive proteins by immunoblot analysis from moth’s wings sensitized patients can be potential allergens. The one at 80 kDa is the major protein, seen in all IgE-reactive patients from group 1 and in none from group 2, yet to be identified. Future studies should be conducted to better characterize these proteins.

Keywords:
Bombyx
Insects
Asthma
Rhinitis
Allergens
Abbrevaitions:
Bm
BmWE
IgE
SDS-PAGE
SPT

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