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Inicio Actas Urológicas Españolas (English Edition) Metalloproteinase 11, potential marker and molecular target in advanced and cast...
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Vol. 41. Issue 6.
Pages 376-382 (July - August 2017)
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Vol. 41. Issue 6.
Pages 376-382 (July - August 2017)
Original article
DOI: 10.1016/j.acuroe.2017.05.004
Metalloproteinase 11, potential marker and molecular target in advanced and castration-resistant prostate cancer. Culture study of peritumoral fibroblasts
Metaloproteinasa 11, potencial marcador y diana molecular en cáncer de próstata avanzado y resistente a la castración. Estudio en cultivo de fibroblastos peritumorales
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J.M. Fernandez-Gomeza,
Corresponding author
jmfergomez@gmail.com

Corresponding author.
, N. Eirob, J.J. García-Rodrígueza, A. Quintás-Blancoa, C. Gonzalez-Ruiz de Leóna, M.L. Perez de Haroa, F. Vizoso-Piñeroc
a Servicio de Urología, Hospital Central de Asturias, Oviedo, Asturias, Spain
b Investigadora, Unidad de Investigación, Fundación Hospital de Jove, Gijón, Asturias, Spain
c Jefe de la Unidad de Investigación, Fundación Hospital de Jove, Gijón, Asturias, Spain
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Table 1. Characteristics of the series.
Abstract
Objective

To analyze the expression of metalloprotein 11 (MMP11) in cultured fibroblasts obtained from human prostate tumors with different clinical and pathological characteristics.

Material and methods

For this study we analyzed samples of transrectal prostate biopsies from tumors with different characteristics, treated with or without androgen deprivation (AD). After optimization of the culture method, fibroblasts were isolated and cultured to perform the study (PCR) of MMP11 mRNA.

Results

Finally, 37 cases were studied: 5 samples of benign prostatic hyperplasia, 14 cases with localized neoplasms (7 high-risk according to the D’Amico classification), 5 with metastasic tumors (bone metastases), and 13 treated with AD therapy, of which 6 fulfilled the requirements to be defined as resistant to castration. In tumors without AD therapy, MMP11 expression was significantly higher (p=0.001) in fibroblasts of higher grade tumors. A significant (p=0.001) correlation was found between PSA and expression of MMP11 in fibroblast s and a significant increase of MMP11 expression in metastatic tumors. In tumors with AD therapy, a significantly greater expression of MMP11 was observed in resistant to castration patients than in those sensitive to castration (p=0.003).

Conclusion

In advanced prostate tumors or in stages of increased tumor aggressiveness, the production of MMP11 by fibroblasts is significantly greater than in non-metastatic tumors or in AD sensitive tumors.

Keywords:
Metalloproteinases
Prostate cancer
Cell cultures
Stroma
Resumen
Objetivo

Analizar el comportamiento de la metaloprotesa 11 (MMP11) en fibroblastos cultivados procedentes de tumores prostáticos humanos con diferentes características clinicopatológicas.

Material y métodos

Para este estudio se analizaron muestras de biopsias de próstata obtenidas por vía transrectal de tumores con diferentes características, tratados o no con privación androgénica (PA). Tras la optimización del método de cultivo, se aislaron y cultivaron los fibroblastos para realizar el estudio (PCR) del ARNm de MMP11.

Resultados

Se estudiaron finalmente 37 casos: 5 muestras de hiperplasia benigna de próstata, 14 casos con neoplasias localizadas (7 de alto riesgo según la clasificación de D’Amico), 5 con tumores con metástasis óseas y 13 tratados con PA, de los que 6 cumplían los requisitos para ser definidos como resistentes a la castración. En los tumores sin PA, la expresión de MMP11 fue significativamente mayor (p=0,001) en los fibroblastos de tumores de grados más altos. Se encontró una correlación significativa (p=0,001) entre PSA y expresión de MMP11 fibroblástica y un aumento significativo de la expresión de MMP11 en los tumores metastásicos. En los tumores con PA se objetivó una expresión significativamente mayor de MMP11 en pacientes resistentes a la castración que en los sensibles a esta (p=0,003).

Conclusión

En tumores de próstata avanzados o en fases de mayor agresividad tumoral, la producción de MMP11 por los fibroblastos resulta significativamente mayor que en tumores no metastásicos o en fases de sensibilidad a la PA.

Palabras clave:
Metaloproteinasas
Cáncer de próstata
Cultivo celular
Estroma

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