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Vol. 41. Issue 7.
Pages 445-450 (September 2017)
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Vol. 41. Issue 7.
Pages 445-450 (September 2017)
Original article
DOI: 10.1016/j.acuroe.2017.06.003
Experimental murine model of renal cancer
Modelo murino experimental de cáncer renal
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B. Padilla-Fernándeza, M.B. García-Cenadorb, P. Rodríguez-Marcosb, J.F. López-Marcosb, P. Antúnez-Plazac, J.M. Silva-Abuínd, D. López-Montañésb, F.J. García-Criadob, M.F. Lorenzo-Gómezb,
Corresponding author
mflorenzogo@yahoo.es

Corresponding author.
a Servicio de Urología, Hospital Universitario de Canarias, Tenerife, Spain
b Departamento de Cirugía, Universidad de Salamanca, Salamanca, Spain
c Servicio de Anatomía Patológica, Hospital Universitario de Salamanca, Salamanca, Spain
d Servicio de Urología, Hospital San Pedro, Logroño, Spain
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Table 1. Results of the first phase.
Table 2. Results of the second phase.
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Abstract
Introduction

The objective of this study was to determine the reproducibility in a murine model of renal tumors of various histological strains that could be useful for investigating the response to target drugs.

Material and methods

Development and analysis of the “in vivo” model: tumor xenograft of renal cell carcinomas with Balb/c nude athymic mice. Nontumourous human renal tissue was implanted in the interscapular region of 5 mice, chromophobe renal cell carcinoma was implanted in 5 mice (which, after checking its growth, was prepared for implantation in another 10 mice) and Fuhrman grade 2 clear cell renal cell carcinoma (CCRCC) was implanted in 5 mice (which was also subsequently implanted in 10 mice).

We monitored the tumor size, onset of metastases and increase in size and number of tumors. When the size had reached a point greater than or equal to locally advanced or metastatic carcinoma, the animals were euthanised for a pathological and immunohistochemical study and a second phase of implantation.

Results

The subcutaneous xenograft of the healthy tissue did not grow. The animals were euthanised at 6 months and no renal tissue was found. The chromophobe renal cell carcinoma cells grew in the initial phase (100%); however, in the second phase, we observed a chronic lymphomonocyte inflammatory reaction and a foreign body reaction. The CCRCC grew at 5–8 months both in the first and second phase (100%), maintaining the tumor type and grade.

Conclusions

The model with athymic Balb/c nude mice is useful for reproducing CCRCC, with the same histological characteristics and aggressiveness as native human tumors, promoting the development of the second experimental phase.

Keywords:
Renal carcinoma
Experimental model
Validation
Resumen
Introducción

El objetivo fue conocer la reproductibilidad en modelo murino de tumores renales de diferentes estirpes histológicas que podría ser útil para investigar la respuesta a fármacos diana.

Material y métodos

Desarrollo y análisis del modelo in vivo: xenoinjerto tumoral de carcinoma de células renales con ratones atímicos nude Balb/c. Se implanta tejido renal humano no tumoral en la región interescapular de 5 ratones, tumor renal tipo cromófobo en 5 ratones que tras comprobarse su crecimiento se preparó para implante en otros 10 ratones y tumor renal tipo carcinoma renal de células claras (CRCC) Fuhrman 2 en 5 ratones que también se implantó posteriormente en 10 ratones.

Se monitoriza el tamaño tumoral, la aparición de metástasis y el aumento de tamaño y número de las mismas. Cuando alcanza tamaño igual o superior a carcinoma localmente avanzado o metastásico los animales son sacrificados para estudio anatomopatológico, inmunohistoquímico y segunda fase de implante.

Resultados

El xenoinjerto subcutáneo del tejido sano no creció, se sacrificaron a los 6 meses sin hallar tejido renal. El carcinoma renal de células cromófobas creció en la primera fase (100%), pero en la segunda fase se observó reacción inflamatoria crónica linfomonocitaria y a cuerpo extraño. El CRCC creció a los 5-8 meses, tanto en la primera como en la segunda fase (100%), manteniendo el tipo y el grado tumoral.

Conclusiones

El modelo con ratones atímicos nude Balb/c es útil para reproducir CRCC, con las mismas características y agresividad histológica al tumor humano nativo, alentando al desarrollo de la segunda fase experimental.

Palabras clave:
Carcinoma renal
Modelo experimental
Validación

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