[4] Measurement of oxidizability of blood plasma

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The hypothesis that the oxidation of low density lipoproteins (LDL) plays a central role in atherogenesis has received much attention over the past decade. The role of the lipoprotein oxidation in atherogenesis implies that lipoprotein oxidizability by physiological oxidants is of a great importance for this process. The oxidizability of isolated lipoproteins is determined by their content of components directly involved in the oxidation process—namely, oxidizable substrates and antioxidant compounds. This chapter describes a method for the measurement of the oxidizability of lipoproteins in diluted blood plasma and presents data supporting its physiological and clinical relevance. Because lipoprotein oxidation occurring in plasma represents a measure of the oxidation of plasma itself, the lipoprotein oxidizability measured in such a system is further referred to as the oxidizability of blood plasma. Taken together, the data presented in this chapter indicate that the plasma oxidation assay (1) provides information similar to that obtained using the common LDL oxidation assay, (2) upgrades the latter, taking into account the effect of hydrophilic antioxidants on lipoprotein oxidation and characterizing the oxidizability of all plasma lipoproteins, and (3) offers important practical advantages, such as fast and simple sample processing, the low amount of plasma required, and avoidance of artifactual oxidation during lipoprotein isolation.

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