Clinical researchBacterial Reduction in Infected Root Canals Treated With 2.5% NaOCl as an Irrigant and Calcium Hydroxide/Camphorated Paramonochlorophenol Paste as an Intracanal Dressing
Section snippets
Clinical Material
Patients presenting to the endodontic clinic at the School of Dentistry, Estácio de Sá University, Rio de Janeiro, Brazil, for evaluation and treatment of apical periodontitis were recruited for this study. Twelve single-rooted teeth (six maxillary central incisors, two maxillary lateral incisors, one maxillary canine, one mandibular incisor, one mandibular canine, and one mandibular premolar) from 11 patients (8 females and 3 males, aged 12–60 years, mean 35 years) were selected for this study
Results
Of the 12 teeth sampled, 1 showed bacterial growth for the sterility control of the working field and was excluded from the study. Bacteria were found in all initial samples (S1) from the other 11 root canals. The median value of the number of CFUs in the initial samples was 3.02 × 105, ranging from 1.68 × 104 to 3.3 × 107.
After chemomechanical preparation using 2.5% NaOCl as an irrigant, 5 of the 11 canals (45.5%) showed negative culture results. The median number of CFUs in
Discussion
In this study, bacterial elimination in infected root canals promoted by a specific treatment protocol was assessed by means of anaerobic-culturing procedures and 16S rRNA gene sequencing for identification of isolates. Even though several cultivable bacterial species were disclosed by these techniques, it is salient to point out that the endodontic microbiota is often underrepresented by this sort of analysis. About 40% to 55% of the bacteria found in endodontic infections have not yet been
Acknowledgments
The authors would like to express their gratitude to Mr. Fernando A. C. Magalhães for his valuable technical assistance.
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Supported by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), a Brazilian governmental institution.