Clinical researchAntibacterial Efficacy of Chlorhexidine Gluconate Intracanal Medication In Vivo
Section snippets
Study Cohort
Before data collection, the research protocol was approved by the Health Sciences Research Ethics Board. Subjects were recruited from among patients in the Graduate Endodontics Clinic, Faculty of Dentistry, University of Toronto, Canada, in accordance with specific inclusion and exclusion criteria. Included were only premolar or anterior teeth presenting with radiographic evidence of apical periodontitis and with adequate coronal structure to ascertain proper isolation, temporization, and
Percentage of Negative Cultures
The proportions of samples showing no bacterial growth on agar plates (percentage negative culture) are summarized in Table 1. The statistical analysis of differences between samples is presented in Table 2. Not all of the access cavity (AC) samples were negative. The 1A samples all showed bacterial growth (positive culture). There was a significant increase (p < 0.001) in the percentage of negative cultures from the 1A to the 1B samples. The percentage of negative cultures decreased
Discussion
The accuracy of bacterial cultures obtained from root canals has been questioned (30), particularly the possibility of false-positive and -negative results. False-positive results can occur because of contamination during sampling, from salivary leakage, from bacteria retained in cracks and the margins of fillings (3, 30), or from handling of the specimens in the clinic or in the laboratory. False-negative results can occur when bacterial growth is inhibited by antibacterial agents used during
Acknowledgment
This study was supported in part by an Endodontic Research Grant from the American Association of Endodontists Foundation and by the Canadian Academy of Endodontics Endowment Fund.
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