Pre-Clinical InvestigationComparison of Echocardiographic Measurements of Left Ventricular Volumes to Full Volume Magnetic Resonance Imaging in Normal and Diseased Rats
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Animal Use and Care
Animals were obtained and handled as approved by the Institutional Animal Care and Use Committee of the Icahn School of Medicine at Mount Sinai in accordance with the “Principles of Laboratory Animal Care by the National Society for Medical Research and the Guide for the Care and Use of Laboratory Animals” (National Institutes of Health Publication No. 86-23, revised 1996).
Surgical Animal Disease Models
We studied 27 rats. Male Sprague-Dawley rats (200–250 g) underwent surgery as previously described.19 Pressure-overload LV
Heart Rate During Imaging
Heart rate per animal group is shown in Table 1. It was significantly higher on echocardiography compared with CMR (394 ± 53 vs 294 ± 54 beats/min; P < .0001, paired t test).
Concordance and Correlation of LV Volumes and EF by HC Echocardiography and CMR (HC CMR and FV CMR)
Representative images of long-axis and short-axis views of the left ventricle by echocardiography and CMR are shown in Figure 2, from the same sham and post-MI animals, respectively. LV volumes (EDV and ESV) and EF measured by HC echocardiography, HC CMR, and FV CMR are shown in Table 1. Diseased groups demonstrated
Discussion
We demonstrate that an easy-to-use HC 2D echocardiographic model of whole LV volume in rats provides measurements of LV size and function that (1) are well correlated with FV CMR in both normal and diseased animals, (2) fall in the same range as FV CMR absolute measurements, particularly for EDV, and (3) compare favorably with FV CMR in their ability to discriminate between disease models of variable severity. We also show that echocardiography per se underestimates corresponding CMR
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Cited by (19)
Echocardiographic Analysis of Cardiac Function after Infarction in Mice: Validation of Single-Plane Long-Axis View Measurements and the Bi-Plane Simpson Method
2018, Ultrasound in Medicine and BiologyCitation Excerpt :This is in line with the results of our study indicating a moderate correlation between single-plane PSLAX and MRI analysis in a mouse model with ischemia and reperfusion. In addition, Arias et al. (2013) performed a study comparing left ventricular volumes measured by echocardiography with full-volume MRI in normal and diseased rats. In that study, the authors used 2-D echocardiographic views for volume calculations based on a hemisphere-cylinder model.
Comparison of segmental contractility evaluated by transthoracic echocardiogram and cardiac magnetic resonance in patients with acute myocardial infarction
2018, Revista Colombiana de Cardiologia
This work was supported by Leducq Foundation through the Caerus Network; by Centro Nacional de Investigaciones Cardiovasculares through the Cardio-Image program (Dr. Arias); and by National Institutes of Health grants R01 HL093183, HL088434, HL071763, HL080498, HL083156, and P20HL100396 (Dr. Hajjar) and NIH-T32-HL007824 (Dr. Chemaly).