Attenuation of obesity-induced adipose tissue inflammation in C3H/HeJ mice carrying a Toll-like receptor 4 mutation
Section snippets
Materials and methods
Animals. Six-week-old male C3H/HeJ mice which have defective LPS signaling due to a missense mutation in the TLR4 gene and control C3H/HeN mice were purchased from CLEA Japan (Tokyo, Japan). The animals were housed in individual cages in a temperature-, humidity-, and light-controlled room (12 h light and 12 h dark cycle) and allowed free access to water and standard chow (Oriental MF; 362 kcal/100 g, 5.4% energy as fat) (Oriental Yeast, Co., Ltd., Tokyo, Japan), when otherwise noted. In the
Animal studies
We examined the effect of high-fat diet on the metabolic phenotypes of C3H/HeJ mice. Body weight gain was indistinguishable between C3H/HeJ and C3H/HeN mice during the 12-week standard diet and thereafter C3H/HeJ mice weighed slightly more than C3H/HeN mice (Fig. 1A). There was no marked difference in body weight between C3H/HeJ and C3H/HeN mice during a high-fat diet, although C3H/HeJ mice weighed more than C3H/HeN mice in response to 6- to 10-week high-fat diet. The weights of the epididymal
Acknowledgments
We thank Dr. Motohiro Takeya for rat monoclonal anti-mouse F4/80 antibody and the members of the Ogawa Laboratory for helpful discussions. This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and Ministry of Health, Labour and Welfare of Japan, and research grants from Japan Diabetes Foundation, Mishima Kaiun Memorial Foundation, The Novo Nordisk Insulin Study Award, The SKYLARK Food Science
References (20)
- et al.
JNK and tumor necrosis factor-alpha mediate free fatty acid-induced insulin resistance in 3T3-L1 adipocytes
J. Biol. Chem.
(2005) - et al.
Saturated fatty acids, but not unsaturated fatty acids, induce the expression of cyclooxygenase-2 mediated through Toll-like receptor 4
J. Biol. Chem.
(2001) - et al.
Blockade of CCR2 ameliorates progressive fibrosis in kidney
Am. J. Pathol.
(2004) - et al.
Activation of Toll-like receptor 4 is associated with insulin resistance in adipocytes
Biochem. Biophys. Res. Commun.
(2006) - et al.
The perils of portliness: causes and consequences of visceral adiposity
Diabetes
(2000) - et al.
Molecular mechanism of metabolic syndrome X: contribution of adipocytokines adipocyte-derived bioactive substances
Ann. N. Y. Acad. Sci.
(1999) - et al.
Minireview: adiposity, inflammation, and atherogenesis
Endocrinology
(2003) - et al.
Adipose tissue, inflammation, and cardiovascular disease
Circ. Res.
(2005) - et al.
Obesity is associated with macrophage accumulation in adipose tissue
J. Clin. Invest.
(2003) - et al.
Chronic inflammation in fat plays a crucial role in the development of obesity-related insulin resistance
J. Clin. Invest.
(2003)
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