ResearchBasic science: GynecologyPolymorphism in a gene coding for the inflammasome component NALP3 and recurrent vulvovaginal candidiasis in women with vulvar vestibulitis syndrome
Section snippets
Materials and Methods
The study population consisted of 143 women with VVS. The control subjects were 182 sexually active women with no history of vulvodynia; they did not undergo a gynecologic examination. All subjects were white. Patients with VVS were identified as having severe pain confined to the vulvar vestibule on light contact with a cotton swab, intense pain on attempted sexual intercourse or tampon insertion, variable degrees of vestibular erythema, and duration of symptoms for at least 6 months. Other
Results
The 4 CIAS1 alleles were combined into 10 different genotypes (Table 1). The distributions were in Hardy-Weinberg equilibrium. In both patients and control subjects the 12,12 genotype was the most prevalent whereas allele 6 was the least prevalent. The 12,12 genotype was found in a higher percentage of control subjects (47.8%) than in patients with VVS (35.7%) (P = .031). Similarly, the allele 12 frequency was 63.2% in control subjects as opposed to 54.2% in patients with VVS (P = .024).
Comment
Homozygosity for the 12 repeat unit of the CIAS1 length polymorphism was the most prevalent genotype in control women and women with VVS. Because this genotype is associated with the highest level of NALP3 production and NALP3 is the rate-limiting step in inflammasome formation9, 13 it appears that the 12,12 genotype is optimal for inflammasome function. Possession of allele 7, which contains 200 fewer bp than allele 12, may result in formation of an altered NALP3-containing inflammasome or an
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Cite this article as: Lev-Sagie A, Prus D, Linhares IM, et al. Polymorphism in a gene coding for the inflammasome component NALP3 and recurrent vulvovaginal candidiasis in women with vulvar vestibulitis syndrome. Am J Obstet Gynecol 2009;200:303.e1-303.e6.