Elsevier

Virology

Volume 314, Issue 1, 15 September 2003, Pages 9-15
Virology

Regular article
Characterization of a novel single-stranded RNA mycovirus in pleurotus ostreatus

https://doi.org/10.1016/S0042-6822(03)00382-9Get rights and content
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Abstract

A mycovirus, named oyster mushroom spherical virus (OMSV), was isolated from cultivated oyster mushrooms with a severe epidemic of oyster mushroom Die-back disease. OMSV was a 27-nm spherical virus encapsidating a single-stranded RNA (ssRNA) of 5.784 kb with a coat protein of approximately 28.5 kDa. The nucleotide sequence of the virus revealed that its genomic RNA was positive strand, containing 5784 bases with seven open reading frames (ORF). ORF1 had the motifs of RNA-dependent RNA polymerases (RdRp) and helicase. ORF2 encoded a coat protein. ORF3 to 7 could encode putative polypeptides of approximately 12, 12.5, 21, 14.5, and 23 kDa, respectively, but none of them showed significant similarity to any other known polypeptides. The 5′ end of the viral RNA was uncapped and the 3′ end was polyadenylated with 74 bases. Genomic structure and organization and the derived amino acid sequence of RdRp and helicase domain were similar to those of tymoviruses, a plant virus group.

Keywords

ssRNA
Mycovirus
RNA-dependent RNA polymerase
Coat protein
Pleurotus ostreatus

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