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Vol. 16. Núm. 1.
Páginas 40-48 (Enero 2012)
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Vol. 16. Núm. 1.
Páginas 40-48 (Enero 2012)
Acceso a texto completo
Presencia y persistencia de variantes moleculares del ORF E6 y ORF E7 del VPH 58 en mujeres con citología normal que pertenecen a la cohorte de Bogotá, Colombia
Presence and Persistence of HPV 58 Molecular Variations ORF E6 and ORF E7 Among Women With Normal Cytology in the Bogotá, Colombia Cohort
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Óscar Buitrago1, Nicolás Morales1, Carolina Martín1, Antonio Huertas1, Pablo Moreno1, Teresa Martínez2, Mónica Molano1,
Autor para correspondencia
mmolano@cancer.gov.co

Correspondencia: Instituto Nacional de Cancerología, Avenida 1a No. 9-85, Bogotá, Colombia. Tel.:éfono (571) 334 1111 ext. 4205.
, Grupo de Estudio VPH 3
1 Grupo de Investigación en Biología del Cáncer, Instituto Nacional de Cancerología, Bogotá D. C., Colombia
2 Grupo de Investigación Epidemiológica, Instituto Nacional de Cancerología, Bogotá D. C., Colombia
3 Grupo de Estudio VPH: Héctor Posso, Margarita Ronderos, Raúl Murillo, Joaquín Luna, Natasha Ortiz, Gilberto Martínez, Edmundo Mora, Gonzalo Pérez, José María Fuentes, Constanza Gómez, Eva Klaus, Constanza Camargo, Cecilia Tabón, Teodolinda Palacio, Carolina Suárez, Claudia Molina
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Resumen
Objetivo

Analizar la presencia y persistencia de variantes en E6/E7/VPH 58 en muestras de mujeres con infecciones prevalentes por VPH 58, con citología normal, que pertenecen a la cohorte de Bogotá, Colombia.

Métodos

Se utilizaron cepillados cervicales de 34 mujeres VPH 58, con citología normal, pertenecientes a la línea de base de la cohorte, con su respectivo seguimiento. Se amplificó la región E6/E7 del VPH 58 usando los iniciadores E6F1-E7R1 y los iniciadores E7P1-E7P2. Para el análisis de las variantes se utilizó la técnica de secuencia automática directa. La secuencia referencia del VPH 58 se utilizó para comparar las secuencias obtenidas.

Resultados

En 27/34 muestras se lograron detectar variantes de E6/E7 de VPH 58. En total, se detectaron cinco variantes diferentes, dos de ellas nunca antes reportadas (A169/T307/A694/G744/A761 y T307/A694/G744/A761/G763). Los análisis de eliminación mostraron que el 75% de las variantes se habían eliminado antes de los dos años de seguimiento, y todas las variantes ya se habían eliminado a los seis años de seguimiento.

Conclusiones

Dos nuevas variantes se reportaron a escala mundial de gran relevancia en los ámbitos filogenético y epidemiológico.

Palabras clave:
virus del papiloma humano
estudios de seguimientos
variación estructural del genoma
Abstract
Objective

To analyze the presence and persistence of E6/E7 HPV58 variations in women with prevalent HPV 58 infection, with normal cytology, who belong to the Bogotá, Colombia cohort.

Methods

Cervical cytobrush was used on 34 HPV58 women, with normal cytology, who are part of the cohort base line; respective follow was performed. The HPV58 E67/E7 region was broadened by using E6F1-E7R1 and E7P1-E7P2 indicators. Variation analysis was carried out with automatic direct sequencing. HPV58 sequence reference was used to compare the sequences that had been obtained.

Results

In 27/34 samples, E6/E7 variations of HPV58 were successfully detected. A total of five different variations were detected, two of which had never been reported before (A169/T307/A694/G744/A761 and T307/A694/G744/A761/G763). Elimination analysis revealed that 75% of variations had been eliminated within two years of follow up, and that all variation had been eliminated at the end of six years of follow up.

Conclusions

Two new variations of universal phylogenetic and epidemiologic noteworthiness were reported

Key words:
Papilloma human virus
follow-up studies
genomic structural variants
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Copyright © 2012. Instituto Nacional de Cancerología
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