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Vol. 45. Núm. 2.
Páginas 37-44 (Enero 2002)
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Vol. 45. Núm. 2.
Páginas 37-44 (Enero 2002)
Acceso a texto completo
Detección de micrometástasis en el ganglio centinela de pacientes con cáncer infiltrante de mama
Detection of micrometastases in the sentinel node of patients with infiltrating breast cancer
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...
F. Tresserra
Autor para correspondencia
25865ftc@comb.es

Correspondencia: Servicio de Anatomía Patológica. Instituto Universitario Dexeus. P.o de la Bonanova, 69. 08017 Barcelona.
, P.J. Grases
Servicio de Anatomía Patológica, Ginecología y Medicina de la Reproducción. Instituto Universitario Dexeus. Barcelona.
M. Izquierdo*, R. Fábregas*, A. Fernández-Cid*
* Departamento de Obstetricia, Ginecología y Medicina de la Reproducción. Instituto Universitario Dexeus. Barcelona.
Información del artículo
Resumen
Objetivo

La afección de los ganglios linfáticos es el factor pronóstico más importante en las pacientes con cáncer de la mama. El examen del ganglio centinela es una alternativa al estudio completo de la axila

Material y métodos

Se han estudiado 164 ganglios centinela en 100 pacientes con cáncer de mama. Se utilizó un protocolo estándar para el estudio de 21 láminas (cuatro de ellas teñidas con inmunohistoquímica). Se relacionó el número de láminas estudiadas con la positividad o no de los ganglios, y en el primer caso con el tipo de afección. También se tuvo en cuenta el tamaño del tumor y el estado del resto de la linfadenectomía

Resultados

Un total de 52 ganglios centinela fue positivo, 17 de ellos evidenciaron metástasis, 19 micrometástasis y 16 células tumorales aisladas. Todas las metástasis se diagnosticaron en la primera lámina estudiada teñida con hematoxilinaeosina; sin embargo, para el diagnóstico de todas las micrometástasis y de las células tumorales aisladas se precisaron 16 y 19 láminas, respectivamente (cuatro de ellas teñidas con inmunohistoquímica)

Conclusión

Para el diagnóstico de micrometástasis y de células tumorales aisladas se precisa un protocolo que incluya múltiples secciones y técnicas de inmunohistoquímica

Palabras clave:
Ganglio centinela
Metástasis
Estadificación
Inmunohistoquímica
Cáncer de mama
Summary
Objective

Axillary lymph node status is the most important prognostic factor in patients with breast carcinoma. Sentinel lymph node (SLN) biopsy is an alternative to complete axillary dissection

Material and methods

We studied 164 SLN in 100 women with breast cancer. A standardized procotol of 21 slides (four of them stained with immunohistochemistry) was used. The positivity or negativity of these lymph nodes and the type of involvement were related to the number of slides studied. Tumoral size and the status of the nonsentinel axillary lymph node were also evaluated

Results

Fifty-two SLN were positive. Of these, 17 showed metastasis, 19 showed micrometastasis and 16 showed isolated tumor cells. All metastases were diagnosed in the first slide stained with hematoxylin and eosin. For the diagnosis of all micrometastases and isolated tumor cells, 16 and 19 slides (four of them stained with immunohistochemistry) were required, respectively

Conclusion

A multiple sectioning protocol including immunohistochemical techniques is required for the diagnosis of micrometastases and isolated tumor cells

Key words:
Sentinel lymph node
Metastases
Staging
Immunohistochemistry
Breast cancer
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Copyright © 2002. Sociedad Española de Ginecología y Obstetricia
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