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Original article
Efficacy of an ultrasound technique in the recovery of spermatozoa during the investigation of sexual offenses
Eficacia del tratamiento ultrasónico en la recuperación de espermatozoides en la investigación de delitos sexuales
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Ana Nataly Lezcano Martinez, Luz Eliana Giraldo Vasquez, Estefania Vanegas Londoño, Tatiana Espinosa Montoya, Jose Daniel Zuluaga Barrios, Carlos Federico Molina Castaño
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cmolina@tdea.edu.co

Corresponding author.
Tecnológico de Antioquia Institución Universitaria, Medellín, Colombia
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Table 1. Results obtained in sperm cell counts in 3μl and 5μl volumes for 1:10, 1:100, 1:250 and 1:500 dilutions and total semen, using samples subjected to ultrasound and samples not subjected to ultrasound.
Abstract
Objective

To evaluate the efficiency for the recovery of spermatozoa using the standard technique and a technique based on ultrasound.

Materials and methods

An experimental study was carried out to compare two techniques for spermatozoa recovery; (i) the standard technique, based on elution of the evidence in distilled water with a buffer and, (ii) a modified technique that included concentration by centrifugation and ultrasound. The efficiency was based on manual counting of spermatozoa using dilutions 1:1 to 1:500.

Results

The modified technique substantially improved the recovery of spermatozoa. While the standard technique recovered 3.1%, the modified one resulted in a recovery of 17.5% using the 1:10 dilution, which represents a six-fold increase. The modified technique was the only one able to recover spermatozoa in dilutions higher than 1:250.

Conclusion

The implementation of the ultrasonic technique improves the recovery of spermatozoa.

Keywords:
Spermatozoa
Sex offences
Ultrasound
Forensic genetics
Resumen
Objetivo

Evaluar la eficiencia para la recuperación de espermatozoides utilizando una técnica estándar y una técnica basada en ultrasonido.

Materiales y métodos

Se realizó un estudio experimental para comparar 2 técnicas de recuperación de espermatozoides: 1) la técnica estándar, basada en la elución de la evidencia en agua destilada con un solución tampón, y 2) una técnica modificada, que incluyó la concentración por centrifugado y el uso de ultrasonido. La eficacia se basó en el recuento manual de espermatozoides utilizando diluciones de 1:1 a 1:500.

Resultados

La técnica modificada mejoró sustancialmente la recuperación de los espermatozoides. En la dilución 1:10, la técnica estándar recuperó el 3,1%, la modificada recuperó un 17,5%; representando un aumento de 6 veces más en la recuperación. La técnica modificada fue la única capaz de recuperar espermatozoides en diluciones superiores a 1:250.

Conclusión

La implementación de la técnica ultrasónica mejora la recuperación de espermatozoides.

Palabras clave:
Espermatozoides
Delitos sexuales
Ultrasonido
Genética forense

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