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Inicio Allergologia et Immunopathologia The expression of DNMTs is dramatically decreased in peripheral blood mononuclea...
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Vol. 48. Issue 2.
Pages 182-186 (March - April 2020)
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Vol. 48. Issue 2.
Pages 182-186 (March - April 2020)
Original Article
DOI: 10.1016/j.aller.2019.08.003
The expression of DNMTs is dramatically decreased in peripheral blood mononuclear cells of male patients with juvenile idiopathic arthritis
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Afshin Abdi Ghavidela,b, Reza Shiaric,d, Vahideh Hassan-Zadehe, Shirin Farivara,
Corresponding author
s_farivar@sbu.ac.ir

Corresponding author.
a Faculty of Life Sciences and Biotechnology, Shahid Beheshti University G.C., Tehran, Iran
b Student Research Committee, Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
c Department of Pediatrics, Shahid Beheshti University of Medical Sciences, Tehran, Iran
d Mofid Children’s Hospital, Pediatrics Infectious Research Center (PIRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran
e Department of Cell and Molecular Biology, School of Biology, Faculty of Science, University of Tehran, Tehran, Iran
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Table 1. Primer sequences used for gene expression analysis.
Table 2. Demographic and clinical characteristics of enrolled individuals.
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Abstract
Introduction

Juvenile idiopathic arthritis (JIA) is an autoimmune rheumatic disease, which affects primarily the joints in children under 16 years old. The etiology of JIA is yet unknown but research has shown that JIA is a multifactorial disease implicating several genes and environmental factors. Environmental factors affect immune cells via epigenetic mechanisms. One of the most important epigenetic mechanisms is DNA methylation catalyzed by DNA methyltransferases (DNMTs) and usually associated with gene silencing. In this study, we analyzed the expression of three DNA methyltransferases namely DNMT1, DNMT3a and DNMT3b in peripheral blood mononuclear cells (PBMCs) of patients with JIA and compared it with the expression of these genes in healthy young individuals.

Materials and methods

Peripheral blood mononuclear cells of 28 JIA patients and 28 healthy controls were isolated. Total RNA was extracted, cDNA was synthesized and the transcript levels of DNMTs were analyzed by quantitative PCR.

Results

Analysis of DNMT1, DNMT3a and DNMT3b relative gene expression in PBMCs of JIA patients and control individuals shows that the expression of DNMT1 and DNMT3a is reduced significantly by 7 folds and 5.5 folds, respectively, in JIA patients compared to healthy controls. Furthermore, the expression of all three DNMTs were significantly and drastically reduced in young affected males compared to healthy males.

Conclusion

This study shows that the expression of DNMTs is reduced in JIA patients and this reduction is severe in male JIA patients.

Keywords:
JIA
Epigenetics
DNMT1
DNMT3a
DNMT3b
PBMC
Autoimmune diseases
Sex characteristics

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