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Vol. 47. Issue 1.
Pages 2-11 (January - February 2019)
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Vol. 47. Issue 1.
Pages 2-11 (January - February 2019)
Original Article
DOI: 10.1016/j.aller.2018.05.004
The effect of neonatal maternal separation on short-chain fatty acids and airway inflammation in adult asthma mice
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L. Qiana,b,1, L. Luc,1, L. Huanga,b, Q. Wena,b, J. Xiea,b, W. Jinb, H. Lib, L. Jianga,b,
Corresponding author
lijiangw34@aliyun.com

Corresponding author.
a Department of Pediatrics, Zhongda Hospital, Southeast University, Nanjing, Jiangsu 210009, China
b Teaching and Research Section of Pediatrics, Medical College, Southeast University, Nanjing, Jiangsu 210009, China
c Department of Neonatology, Suzhou Municipal Hospital, Suzhou, Jiangsu 210002, China
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Abstract
Background

To investigate neonatal maternal separation (NMS) effects on airway inflammation of asthma and potential mechanism using a mouse model.

Methods

80 Balb/c neonatal male mice were randomly assigned to NMS and non-NMS groups. Feces were collected on PND21, 28, 35 and 42 to analyze microbiota and short-chain fatty acids (SCFAs). Non-NMS group were then divided into control (group A) and asthma groups (group B), while NMS group was assigned to NMS+asthma (group C) and NMS+SCFAs+asthma groups (group D). Inflammatory cells and eosinophils (EOS) in bronchoalveolar lavage fluid (BALF) were assessed. Pathological changes and cytokines in lung tissue were observed. Protein expression of Occludin and E-cadherin in airway epithelial was examined.

Results

The number of S′, diversity index H′ and dominance index D′, as well as content butyric acid in NMS group C were significantly lower than non-NMS group B (p<0.05). Mice in group C had a higher level of inflammatory cells and EOS compared with group A, B and D. EOS moderate infiltration was found in mice of group B, C and D. Mice in group C had significantly higher levels of cytokines and showed slightly increased bronchial epithelium goblet cells and a small amount of visceral secretions. Occludin and E-cadherin expression in lung in B, C and D groups was depressed, and protein level in group C was significantly lower than group B and D.

Conclusions

NMS is associated with exacerbated inflammation of adult asthma by changing intestinal microflora resulting in butanoic acid decline and airway epithelial barrier damage.

Keywords:
Early life stress
Neonatal maternal separation
Intestinal microflora
Asthma

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