Regístrese
Buscar en
Allergologia et Immunopathologia
Toda la web
Inicio Allergologia et Immunopathologia Osteoprotegerin mediate RANK/RANKL signaling inhibition eases asthma inflammator...
Journal Information
Vol. 47. Issue 2.
Pages 179-184 (March - April 2019)
Share
Share
Download PDF
More article options
ePub
Vol. 47. Issue 2.
Pages 179-184 (March - April 2019)
Original Article
DOI: 10.1016/j.aller.2018.06.006
Osteoprotegerin mediate RANK/RANKL signaling inhibition eases asthma inflammatory reaction by affecting the survival and function of dendritic cells
Visits
15
X. Yanga,f, X. Wanga,f, M. Chib,c,f, M. Zhanga, H. Shana, Q.-H. Zhanga, J. Zhanga, J. Shia, J.-Z. Zhangd, R.-M. Wue, Y.-L. Lia,
Corresponding author
yalili2008@163.com

Corresponding author.
a Department of Respiratory Medicine, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi‘an, China
b Department of Pediatrics, BaYi Children's Hospital of the PLA Army General Hospital, Beijing, China
c The Clinical Medical College of the PLA Army, Second Military Medical University of People's Liberation Army, Shanghai, China
d Department of Respiratory Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi‘an, China
e Department of Respiratory Medicine, Shaanxi Provincial People's Hospital, Xi‘an, China
This item has received
15
Visits
Article information
Abstract
Full Text
Bibliography
Download PDF
Statistics
Figures (3)
Show moreShow less
Tables (1)
Table 1. Inflammatory pathology score of mouse lung tissue.
Abstract
Introduction

Asthma is a chronic inflammatory, heterogeneous airway disease affecting millions of people around the world. Dendritic cells (DCs) are considered the most important antigen-presenting cell in asthma airway inflammatory reaction. But whether osteoprotegerin (OPG) mediate RANK/RANKL signaling inhibition influences asthma development by affecting the survival and function of DCs remains unclear. In this study, we assessed the effects of OPG on DCs and asthma.

Material and methods

BALB/c mice immunized with ovalbumin (OVA) were challenged thrice with an aerosol of OVA every second day for eight days. Dexamethasone (1.0mg/kg) or OPG (50μg/kg) was administered intraperitoneally to OVA-immunized BALB/c mice on day 24 once a day for nine days. Mice were analyzed for effects of OPG on asthma, inflammatory cell infiltration and cytokine levels in lung tissue. The expression of RANK and β-actin was detected by Western Blot. DCs were isolated from mouse bone morrow. Cell survival was assessed by cell counting. The content of IL-12 was detected by ELISA.

Results

Results showed that OVA increased the number of inflammatory factors in BALF, elevated lung inflammation scores in mice. OPG reversed the alterations induced by OVA in the asthmatic mice. OPG inhibited the survival and function of DC via inhibition of RANK/RANKL signaling.

Conclusions

This research proved inhibition of RANK/RANKL signaling by OPG could ease the inflammatory reaction in asthma, providing new evidence for the application of OPG on asthma.

Keywords:
Asthma
Osteoprotegerin
RANK/RANKL
Dendritic cells
Abbreviations:
APCs
BALF
BCA
BMDCs
cDCs
DCs
DXM
ELISA
HRP
IFN-γ
IL
mDCs
LPS
MHC-II
OPG
pDCs
PI3K
PVDF
SPF

Article

These are the options to access the full texts of the publication Allergologia et Immunopathologia
Subscriber
Subscriber

If you already have your login data, please click here .

If you have forgotten your password you can you can recover it by clicking here and selecting the option “I have forgotten my password”
Subscribe
Subscribe to

Allergologia et Immunopathologia

Contact
Phone for subscriptions and reporting of errors
From Monday to Friday from 9 a.m. to 6 p.m. (GMT + 1) except for the months of July and August which will be from 9 a.m. to 3 p.m.
Calls from Spain
932 415 960
Calls from outside Spain
+34 932 415 960
E-mail
Article options
Tools
es en pt

¿Es usted profesional sanitario apto para prescribir o dispensar medicamentos?

Are you a health professional able to prescribe or dispense drugs?

Você é um profissional de saúde habilitado a prescrever ou dispensar medicamentos

es en pt
Política de cookies Cookies policy Política de cookies
Utilizamos cookies propias y de terceros para mejorar nuestros servicios y mostrarle publicidad relacionada con sus preferencias mediante el análisis de sus hábitos de navegación. Si continua navegando, consideramos que acepta su uso. Puede cambiar la configuración u obtener más información aquí. To improve our services and products, we use "cookies" (own or third parties authorized) to show advertising related to client preferences through the analyses of navigation customer behavior. Continuing navigation will be considered as acceptance of this use. You can change the settings or obtain more information by clicking here. Utilizamos cookies próprios e de terceiros para melhorar nossos serviços e mostrar publicidade relacionada às suas preferências, analisando seus hábitos de navegação. Se continuar a navegar, consideramos que aceita o seu uso. Você pode alterar a configuração ou obter mais informações aqui.