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Inicio Allergologia et Immunopathologia Modulation effect of Lactobacillus acidophilus KLDS 1.0738 on gut microbiota and...
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Vol. 48. Issue 2.
Pages 149-157 (March - April 2020)
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Vol. 48. Issue 2.
Pages 149-157 (March - April 2020)
Original Article
DOI: 10.1016/j.aller.2019.06.002
Modulation effect of Lactobacillus acidophilus KLDS 1.0738 on gut microbiota and TLR4 expression in β-lactoglobulin-induced allergic mice model
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W.-w. Ni, Q.-m. Zhang, X. Zhang, Y. Li, S.-s. Yu, H.-y. Wu, Z. Chen, A.-l. Li
Corresponding author
aili-mail@163.com

Corresponding authors.
, P. Du
Corresponding author
rocgin@126.com

Corresponding authors.
, C. Li
Key Laboratory of Dairy Science, Food Science College, Northeast Agriculture University, Northeast Agriculture University, Harbin, China
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Tables (2)
Table 1. Effects of Lap KLDS 1.0738 on allergic symptoms of β-Lg sensitized mice.
Table 2. Illumina sequencing data summary.
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Additional material (1)
Abstract
Objectives

β-lactoglobulin (β-Lg)-sensitized mice model was employed to investigate the correlation between Lactobacillus acidophilus KLDS 1.0738 (Lap KLDS 1.0738) modulating gut microbiota and inducting Toll-like receptors (TLRs) expression.

Methods

The alterations of mice fecal microbiota were analyzed by 16S rRNA gene sequencing. The serum cytokines production and TLR4/NF-κB mRNA expression in the colon tissues were measured by ELISA kit and quantitative RT-PCR, respectively.

Results

The results showed that Lap KLDS 1.0738 pretreatment attenuated β-Lg-induced hypersensitivity, accompanied with a diminished expression of TLR4/NF-κB signaling. Moreover, oral administration of Lap KLDS 1.0738 improved the richness and diversity of fecal microbiota, which was characterized by fewer Proteobacteria phylum and Helicobacteraceae family, and higher Firmicutes phylum and Lachnospiraceae family than allergic group. Notably, TLR4/NF-κB expression was positively correlated with the family of Helicobacteraceae in allergic group, but negatively correlated with the family of Lachnospiraceae, Ruminococcaceae and anti-inflammatory cytokines level. A significant positive correlation was observed between TLR4/NF-κB expression and the production of histamine, total IgE and pro-inflammatory cytokines.

Conclusions

Intake of Lap KLDS 1.0738 can influence the gut bacterial composition, which might result in recognizing TLRs signaling so as to inhibit allergic response.

Keywords:
Lactobacillus acidophilus
β-lactoglobulin allergy
Gut microbiota
TLR4
16S rRNA gene sequencing

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