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Inicio Allergologia et Immunopathologia Bacterial extract (OM-85) with human-equivalent doses does not inhibit the devel...
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Vol. 44. Issue 6.
Pages 504-511 (November - December 2016)
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Vol. 44. Issue 6.
Pages 504-511 (November - December 2016)
Original Article
DOI: 10.1016/j.aller.2016.04.010
Bacterial extract (OM-85) with human-equivalent doses does not inhibit the development of asthma in a murine model
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A. Rodriguesa, L.P. Gualdia, R.G. de Souzaa, M.H.M. Vargasa, N.K. Nuñeza, A.A. da Cunhaa,
Corresponding author
aline.cunha@pucrs.br

Corresponding author.
, M.H. Jonesb, L.A. Pintoa, R.T. Steina, P.M. Pitreza
a Laboratory of Pediatric Respirology, Infant Center, Institute of Biomedical Research, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil
b Laboratory of Respiratory Physiology, Infant Center, Institute of Biomedical Research, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil
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Table 1. Doses of OM-85 considering human-equivalent doses used in our protocol, when compared to previous studies, and to the package leaflet commercially available.
Abstract
Background

OM-85 is an immunostimulant bacterial lysate, which has been proven effective in reducing the number of lower airways infections. We investigated the efficacy of the bacterial lysate OM-85 in the primary prevention of a murine model of asthma.

Methods

In the first phase of our study the animals received doses of 0.5μg, 5μg and 50μg of OM-85 through gavage for five days (days −10 to −6 of the protocol), 10 days prior to starting the sensitisation with ovalbumin (OVA), in order to evaluate the results of dose–response protocols. A single dose (5μg) was then chosen in order to verify in detail the effect of OM-85 on the pulmonary allergic response. Total/differential cells count and cytokine levels (IL-4, IL-5, IL-13 and IFN-γ) from bronchoalveolar lavage fluid (BALF), OVA-specific IgE levels from serum, lung function and lung histopathological analysis were evaluated.

Results

OM-85 did not reduce pulmonary eosinophilic response, regardless of the dose used. In the phase protocol using 5μg/animal of OM-85, no difference was shown among the groups studied, including total cell and eosinophil counts in BALF, serum OVA-specific IgE, lung histopathologic findings and lung resistance. However, OM-85 decreased IL-5 and IL-13 levels in BALF.

Conclusions

OM-85, administered in early life in mice in human-equivalent doses, does not inhibit the development of allergic pulmonary response in mice.

Keywords:
Asthma
OM-85
Inflammation
Mice

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