TY - JOUR T1 - Direct identification of pneumococcal serotypes in blood cultures by a PCR-reverse-hybridisation technique JO - Enfermedades Infecciosas y Microbiología Clínica (English Edition) T2 - AU - Sanz,Juan Carlos AU - de Luis,Ruth AU - del Río,Susana AU - Gamen,Susana AU - Cercenado,Emilia AU - Orellana,Maria Angeles AU - Yuste,Jose SN - 2529993X M3 - 10.1016/j.eimce.2019.04.022 DO - 10.1016/j.eimce.2019.04.022 UR - https://www.elsevier.es/en-revista-enfermedades-infecciosas-microbiologia-clinica-english-428-articulo-direct-identification-pneumococcal-serotypes-in-S2529993X20300587 AB - IntroductionThe objective of this study was to assess the performance of a technique (S. PneumoStrip test) based on PCR followed by reverse strip hybridisation for the detection of Streptococcus pneumoniae serotypes directly in blood culture vials. MethodsOne hundred and ten (110) pairs of isolated strains and their corresponding original blood cultures vials were studied in parallel. Pure isolated strains were conventionally serotyped using latex agglutination and the Quellung reaction. The S. PneumoStrip test was carried out directly in the original blood culture samples. ResultsIn 102 cases (92.7%), results of the serotype obtained by Quellung coincided with their corresponding original blood cultures typed by S. PneumoStrip. ConclusionsS. PneumoStrip test is a good alternative technique for direct pneumococcal serotyping in blood culture clinical samples. ER -