TY - JOUR T1 - The impact of obesity in the cardiac lipidome and its consequences in the cardiac damage observed in obese rats JO - Clínica e Investigación en Arteriosclerosis (English Edition) T2 - AU - Marín-Royo,Gema AU - Martínez-Martínez,Ernesto AU - Gutièc)rrez,Beatriz AU - Jurado-López,Raquel AU - Gallardo,Isabel AU - Montero,Olimpio AU - Bartolomèc),Mª Visitación AU - Román,Josèc) Alberto San AU - Salaices,Mercedes AU - Nieto,María Luisa AU - Cachofeiro,Victoria SN - 25299123 M3 - 10.1016/j.artere.2017.07.006 DO - 10.1016/j.artere.2017.07.006 UR - https://www.elsevier.es/en-revista-clinica-e-investigacion-arteriosclerosis-english-415-articulo-the-impact-obesity-in-cardiac-S2529912318000062 AB - AimsTo explore the impact of obesity on the cardiac lipid profile in rats with diet-induced obesity, as well as to evaluate whether or not the specific changes in lipid species are associated with cardiac fibrosis. MethodsMale Wistar rats were fed either a high-fat diet (HFD, 35% fat) or standard diet (3.5% fat) for 6 weeks. Cardiac lipids were analyzed using by liquid chromatography-tandem mass spectrometry. ResultsHFD rats showed cardiac fibrosis and enhanced levels of cardiac superoxide anion (O2), HOMA index, adiposity, and plasma leptin, as well as a reduction in those of cardiac glucose transporter (GLUT 4), compared with control animals. Cardiac lipid profile analysis showed a significant increase in triglycerides, especially those enriched with palmitic, stearic, and arachidonic acid. An increase in levels of diacylglycerol (DAG) was also observed. No changes in cardiac levels of diacyl phosphatidylcholine, or even a reduction in total levels of diacyl phosphatidylethanolamine, diacyl phosphatidylinositol, and sphingomyelins (SM) was observed in HFD, as compared with control animals. After adjustment for other variables (oxidative stress, HOMA, cardiac hypertrophy), total levels of DAG were independent predictors of cardiac fibrosis while the levels of total SM were independent predictors of the cardiac levels of GLUT 4. ConclusionsThese data suggest that obesity has a significant impact on cardiac lipid composition, although it does not modulate the different species in a similar manner. Nonetheless, these changes are likely to participate in the cardiac damage in the context of obesity, since total DAG levels can facilitate the development of cardiac fibrosis, and SM levels predict GLUT4 levels. ER -