TY - JOUR T1 - High frequency of ancestral allele of the TJP1 polymorphism rs2291166 in Mexican population, conformational effect and applications in surgery and medicine JO - Cirugía y Cirujanos (English Edition) T2 - AU - Ramirez-Garcia,Sergio Alberto AU - Flores-Alvarado,Luis Javier AU - Topete-González,Luz Rosalba AU - Charles-Niño,Claudia AU - Mazariegos-Rubi,Manuel AU - Dávalos-Rodríguez,Nory Omayra SN - 24440507 M3 - 10.1016/j.circen.2015.12.013 DO - 10.1016/j.circen.2015.12.013 UR - https://www.elsevier.es/en-revista-cirugia-cirujanos-english-edition--237-articulo-high-frequency-ancestral-allele-tjp1-S2444050715001606 AB - BackgroundTJP1 gene encodes a ZO-1 protein that is required for the recruitment of occludins and claudins in tight junction, and is involved in cell polarisation. It has different variations, the frequency of which has been studied in different populations. In Mexico there are no studies of this gene. These are required because their polymorphisms can be used in studies associated with medicine and surgery. Therefore, the aim of this study was to estimate the frequency of alleles and genotypes of rs2291166 gene polymorphism TJP1 in Mexico Mestizos population, and to estimate the conformational effect of an amino acid change. Material and methodsA total of 473 individuals were included. The rs2291166 polymorphism was identified PASA PCR-7% PAGE, and stained with silver nitrate. The conformational effect of amino acid change was performed in silico, and was carried out with servers ProtPraram Tool and Search Database with Fasta. ResultsThe most frequent allele in the two populations is the ancestral allele (T). A genotype distribution similar to other populations was found. The polymorphism is in Hardy–Weinberg, p>0.05. Changing aspartate to alanine produced a conformational change. ConclusionsThe study reveals a high frequency of the ancestral allele at rs2291166 polymorphism in the Mexican population. ER -