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FI 2016

1,125
© Thomson Reuters, Journal Citation Reports, 2016

Indexada en:

Current Contents/Clinical Medicine, Journal Citation Reports, SCI-Expanded, Index Medicus/Medline, Excerpta Medica/EMBASE, IBECS, IME, MEDES, PASCAL, SCOPUS, ScienceDirect

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  • Factor de Impacto: 1,125(2016)
  • 5-años Factor de Impacto: 1,292
  • SCImago Journal Rank (SJR):0,221
  • Source Normalized Impact per Paper (SNIP):0,385

© Thomson Reuters, Journal Citation Reports, 2016

Med Clin 2001;117:416-8 - DOI: 10.1016/S0025-7753(01)72133-1
Sensibilidad disminuida a vancomicina en cepas isogénicas de Staphylococcus aureus aisladas del mismo paciente
Decreasing susceptibility to vancomycin in isogenic Staphylococcus aureus strains isolated from a single patient
Álvaro Pascualaa, , Jesús Rodríguez-Bañob, Encarnación Ramírez de Arellanoa, José Molacc, Luis Martínez-Martíneza
a Departamento de Microbiología
b Sección de Enfermedades Infecciosas
c Servicio de Nefrología. Hospital Universitario Virgen Macarena. Sevilla
Recibido 01 febrero 2001, Aceptado 03 julio 2001
Resumen
Background

We aimed at evaluating the microbiological characteristics and clinical implication of 7 isogenic strains of Staphylococcus aureus with decreased susceptibility to vancomycin.

Subject and method

A patient with nosocomial trivalvular endocarditis due to methicillin-resistant S. aureus had positive blood cultures for 8 weeks despite antimicrobial therapy with vancomycin (normal serum levels) and rifampicin. The patient rejected surgical treatment but the process cured with antimicrobial therapy. The epidemiological relationship of strains was evaluated by pulsed field gel electrophoresis. The susceptibility to vancomycin was evaluated by microdilution and E-test using two different inocula. The activity of oxacillin, ampicillin and cephalotin was evaluated in Mueller Hinton (MH) agar containing different concentrations of vancomycin (0.06 to 4 mg/l). Heteroresistant subpopulations were determined in both MH and brain heart infussion agar containing vancomycin (0.5 to 512 mg/l).

Results

All seven strains belonged to the same clone. Vancomycin MIC ranged from 1 mg/l (isolates 1 to 5) to 4 mg/l (isolates 6 and 7). Isolates 6 and 7 displayed differences in pigmentation and slower growth rates. The susceptibility of these isolates to betalactams in MH containing different concentrations of vancomycin was greater than that in controls. Subpopulations heteroresistant to vancomycin were not detected.

Conclusion

The decreased susceptibility of the isogenic S. aureus strains evaluated in this study was not due to heteroresistance and did not cause therapeutic failure.

Palabras clave
Staphylococcus aureus, Vancomicina, GISA
Keywords
Staphylococcus aureus, Vancomycin, GISA
El Texto completo solo está disponible en PDF
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