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  • SCImago Journal Rank (SJR):0,311
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Acta Otorrinolaringol Esp 2004;55:139-44 - DOI: 10.1016/S0001-6519(04)78497-6
Extracción de adn con resina chelex en el análisis de la amplificación oncogénica en carcinomas de cabeza y cuello
DNA extraction using chelex resin for the oncogenic amplification analysis in nead and neck tumours
L.A. García González,a, , J.P. Rodrigo Tapia**, P. Sánchez Lazo***, S. Ramos***, C. Suárez Nieto**
* Servicio de orl. hospital carmen y severo ochoa. asturias
** Servicio de orl. hospital central de asturias. instituto de oncología. universidad de oviedo
*** Departamento de biología molecular. instituto de oncología. universidad de oviedo
Recibido 10 diciembre 2003, Aceptado 29 enero 2004

La extracción de ADN de tejido tumoral puede ser el proceso más laborioso y complejo en la amplificación de ADN mediante PCR cuando se utiliza el procedimiento con fenol-cloroformo. Comparamos este método de extracción extenso, lento y caro con otras dos técnicas basadas en el uso de resina Chelex-100. Esta resina quelante ha sido utilizada para la extracción de ADN de diferentes tejidos para su uso con la PCR. Estos procedimientos son simples, rápidos y no requieren múltiples pasos. En este trabajo comparamos la extracción de ADN procedente de 30 carcinomas epidermoides de cabeza y cuello utilizando la precipitación con solventes orgánicos, resina Chelex-100 con y sin procesamiento previo con proteinasa K. Los resultados evidencian que el procedimiento con proteinasa K y resina Chelex-100 es un método tan eficaz como la precipitación con fenol-cloroformo


DNA extraction from tissues can be the most laborious and complex step in amplifying DNA by PCR when phenol-choroform procedure is used. We compare this lengthy, slow and expensive extraction method with other two based in the use of Chelex-100 resin. This chelating resin has been applied for extracting DNA from different tissues to use with the PCR. These procedures are simple, rapid and do not require multiple steps. In this study we compared DNA extraction from 30 head and neck squamous cell carcinomas (HNSCC) using organic solvent precipitation, Chelex 100 resin with and without proteinase K pretreatment. The results show that proteinase K-Chelex 100 procedure is as efficient as the phenol-chloroform one

Palabras clave
Tumores de cabeza y cuello, Factores de crecimiento fibroblástico, Extracción de ADN, Amplificación de oncogenes, Reacción en cadena de polimerasa, Resina Chelex-100
Key words
Head cancer, Neck cancer, Fibroblastic growth factors, DNA extraction, Oncogene amplification, Polymerase chain reaction, Chelex 100-resin
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